Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Asian Journal of Animal Sciences
  Year: 2016 | Volume: 10 | Issue: 1 | Page No.: 15-28
DOI: 10.3923/ajas.2016.15.28
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Evaluation of Low Level Laser-Activated Stromal Vascular Fraction as a Single Procedure for Treatment of Experimental Chondral Defects

Ahmed N. Abdallah, Ashraf A. Shamaa , Omar S. El- Tookhy and Eman M. Abd El- Mottaleb

Abstract:
Regenerative therapies offer attractive alternatives for the treatment of chondral defects. Adipose-Derived Mesenchymal Stem Cells (AD-MSCs) found in the Stromal Vascular Fraction (SVF) allow the development of one-step surgical procedure by their abundant availability and a minimal invasive technique. Low Level Laser Irradiation (LLLI) of stem cells showed improved results on stem cell growth and proliferation. The aim of this study was to evaluate the treatment of chondral defects using single injection of laser activated SVF in a Platelet Rich Plasma (PRP) vehicle. Chondral defects were created surgically in the femoral condyle of 9 dogs, autologous adipose tissue was harvested from the abdomen and SVF was isolated, added to the PRP, laser irradiated and injected intraarticularly in the right joint. The left joint was injected normal saline as control negative. Evaluation of the treatment was done by physical examination, radiology and histopathology. Treated joints showed marked degree of cartilage regeneration and restoration of chondral histomorphological picture on the contrary of the control joints that showed deterioration over time and defect filling with only fibrous tissue forming a fibrocartilage at the end of 6 months period. The obtained results proved that the use of low level laser activated adipose derived stem cells is a safe, feasible technique as a single step surgical procedure and a very promising option for treatment of chondral defects.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Ahmed N. Abdallah, Ashraf A. Shamaa, Omar S. El- Tookhy and Eman M. Abd El- Mottaleb, 2016. Evaluation of Low Level Laser-Activated Stromal Vascular Fraction as a Single Procedure for Treatment of Experimental Chondral Defects. Asian Journal of Animal Sciences, 10: 15-28.

DOI: 10.3923/ajas.2016.15.28

URL: https://scialert.net/abstract/?doi=ajas.2016.15.28

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 

Curve Bottom