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Asian Journal of Animal Sciences
  Year: 2015 | Volume: 9 | Issue: 2 | Page No.: 73-79
DOI: 10.3923/ajas.2015.73.79
 
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Application of Sequence-Characterized Amplified Regions to the Detection of Self-biting Behavior in the Blue Fox
Zongyue Liu, Erjun Ren, Shiyong Wang, Xiumei Xing and Fuhe Yang

Abstract:
The aim of this study was to use defined molecular markers to diagnose the self-biting behavior in blue fox. A single RAPD marker, S414, amplified a specific band of 768 bp in the stereotyped behavior group and this amplicon was designated SRS414. The sequences of SRS414 exhibited a 70% similarity to the Macaca mulatta MHC class I region. The RAPD marker was converted into a Sequence-Characterized Amplified Regions (SCAR) marker via cloning and sequencing of the RAPD amplification product and subsequent design of corresponding 18-mer oligonucleotide primers. The RAPD and SCAR markers were then validated in the two blue fox groups. The results of a χ2 test revealed a significant difference (p<0.05) between the detection rate of the two groups. This indicated that SCAR-S414 can be used as a positive marker to detect on farm stereotyped behavior.
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How to cite this article:

Zongyue Liu, Erjun Ren, Shiyong Wang, Xiumei Xing and Fuhe Yang, 2015. Application of Sequence-Characterized Amplified Regions to the Detection of Self-biting Behavior in the Blue Fox. Asian Journal of Animal Sciences, 9: 73-79.

DOI: 10.3923/ajas.2015.73.79

URL: https://scialert.net/abstract/?doi=ajas.2015.73.79

 
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