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Asian Journal of Animal Sciences

Year: 2015 | Volume: 9 | Issue: 2 | Page No.: 57-64
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Authors

Sutarno

A.D. Setyawan

A.J. Lymbery

Keywords

Research Article

Genetic Diversity of Five Indonesian Native Cattle Breeds at Microsatellite Loci

Sutarno, A.D. Setyawan and A.J. Lymbery
The long term objective of this study is to assist in the conservation of Indonesian native cattle breeds which are currently facing serious threats as a result of small population sizes, genetic drift and replacement by imported breeds. The study was conducted to evaluate the genetic diversity within and among five breeds of Indonesian native cattle from Madura, Lombok, Sumbawa, Aceh and Java. Five microsatellite loci were amplified in 40 animals from each breed. A total of 74 alleles were detected over all loci in all of the breeds. The mean observed number of alleles was 3.2 across all loci, with a mean observed heterozygosity of 0.77 and mean expected heterozygosity of 0.53. The observed heterozygote excess may be a result of outcrossing among breeds. Madura cattle were the most genetically distinct of all the breeds studied.
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