Identification and Probiotic Characteristics of Lactic Acid Bacteria Isolated from Indonesian Local Beef


	Asian Journal of Animal Sciences
	Year: 2015 \| Volume: 9 \| Issue: 1 \| Page No.: 25-36 DOI: 10.3923/ajas.2015.25.36

Identification and Probiotic Characteristics of Lactic Acid Bacteria Isolated from Indonesian Local Beef

Irma Isnafia Arief, Betty Sri Laksmi Jenie, Made Astawan, Kazuhito Fujiyama and Arief Budi Witarto

Abstract: Functional foods are food that can improve health by providing benefit beyond that of the basic nutritional value. Probiotics are included as component of functional foods. Probiotics are living microorganisms which when administered in adequate amounts confer a healthy benefit on the host. The research aim was to identify and characterize as probiotic of Indonesian Lactic Acid Bacteria (LAB) that isolated from fresh beef derived from traditional market around Bogor, West Java, Indonesia. The results showed that the 16S rRNA gene could be amplified by PCR (Polymerase Chain Reaction) to get single band with designed primer. Identification by 16S rRNA gene sequencing of 20 isolates showed that 11 isolates were identified as Lactobacillus plantarum, 6 isolates as Lactobacillus acidophilus, 2 isolates as Pediococcus pentosaceus and 1 isolate as Enterococcus faecium. The twenty LAB isolates showed different ability to survive under simulated gastrointestinal conditions. Only 10 isolates could survive well at pH 2.0 (survival rate over than 50%) and the population increased at pH 2.5, 3.2 and 7.2. The ten LAB isolates i.e., 7 strains of L. plantarum 1B1, 1A5, 2D1, 1C4, 2B1, 2B2, 2C12 and 3 strains of L. acidophilus 2B4, 2C2 and 1A32 demonstrated other important probiotic characteristics including survival ability at 0.5% bile salt, antibacterial activities and had good coaggregationcapabilities against the tested pathogenic bacteria (Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 14028, enteropathogenic Escherichia coli/EPEC and Staphylococcus aureus ATCC 25923) by in vitro analysis.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Lactobacillus plantarum IIA-1A5 Fermentation Patterns by Using whey, buttermilk and Whey Enriched by Skimmed Milk as Growth Media

•	Isolation and Molecular Identification of Food Grade Lactic Acid Bacteria and Their Antifungal Activity

•	Isolation and Identification of Lactic Acid Bacteria from Okara and Evaluation of Their Potential as Candidate Probiotics

•	Application of Purified Bacteriocin from Lactobacillus plantarum IIA-1A5 as a Bio-preservative of Beef Sausage

•	Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Burkina Faso Fermented Milk

How to cite this article:

Irma Isnafia Arief, Betty Sri Laksmi Jenie, Made Astawan, Kazuhito Fujiyama and Arief Budi Witarto, 2015. Identification and Probiotic Characteristics of Lactic Acid Bacteria Isolated from Indonesian Local Beef. Asian Journal of Animal Sciences, 9: 25-36.

DOI: 10.3923/ajas.2015.25.36

URL: https://scialert.net/abstract/?doi=ajas.2015.25.36

COMMENT ON THIS PAPER

	Full Name:
	E-mail:
	Comments:

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		FSTA
		Google Scholar
		ISI Web of Knowledge
		Thomson ISI

Follow @scialert

Science Alert