Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Asian Journal of Animal Sciences
  Year: 2014 | Volume: 8 | Issue: 3 | Page No.: 98-105
DOI: 10.3923/ajas.2014.98.105
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Sex Identification in Ruminant using SRY and Microsatellite Markers

Zhang Xiu-Hua, Fan Xue-Hui, Wangjing and Wu-Dengjun

Abstract:
Multiplex PCR amplification of four microsatellite markers and the SRY was used to identify the sex of the ruminant in the study. Result indicates that the SRY primers are highly specific and can be used as the main basis for sex-identification. The markers of MCM158 and MAF45 are not suitable for sex identification because their low specificity. If the PCR-products of MILVET09 and AE25 are positive but the SRY is negative, then the individual is female. The gene type of MILVET09 and AE25 dose not affect the sex determination of the female. The gene type of MCM158 and MAF45 dose not affect the result of sex-identification, but they can further confirm the male identity. In conclusion, if the PCR-products of SRY and four microsatellite markers are present simultaneously and the gene type of MILVET09 and AE25 is homozygous, then the individual is determined to be normal male, if the PCR-products of four microsatellite markers are present, but the SRY gene is not, then the individual is female. And the gene type of MILVET09 and AE25 does not affect the result of the sex-identification of tested individuals.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Zhang Xiu-Hua, Fan Xue-Hui, Wangjing and Wu-Dengjun , 2014. Sex Identification in Ruminant using SRY and Microsatellite Markers. Asian Journal of Animal Sciences, 8: 98-105.

DOI: 10.3923/ajas.2014.98.105

URL: https://scialert.net/abstract/?doi=ajas.2014.98.105

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 

Curve Bottom