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Asian Journal of Animal Sciences
  Year: 2014 | Volume: 8 | Issue: 3 | Page No.: 79-85
DOI: 10.3923/ajas.2014.79.85
 
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Fusion Competence of Interspecies Nuclear Transfer Embryos Reconstructed from Pedicle Periosteum Cell of Sika Deer (Cervus nippon) and Oocyte of Bovine

Shiyong Wang, Junjun Zheng, Yuechun Yang, Zongyue Liu, Miao Yu, Chunyi Li and Fuhe Yang

Abstract:
The electrical fusion procedure used in Nuclear Transfer (NT) is one of the critical factors affecting the efficiency of animal cloning. The objective of this study was to compare the fusion competence of sika deer (Cervus nippon) and bovine interspecies nuclear transfer (iSCNT) in different electrical fusion parameters. The NT-embryos were obtained by transfer of the pedicle periosteum cell of deer at the 4th passage into the enucleated metaphase II (M II) bovine oocytes. As results shown, the percentage of couplets successfully fused at 2.4 kV cm-1 was the highest (57.2% vs 31.5, 41.0 and 45.7%, p<0.05). The percentage of couplets successfully and reconstructed embryos cleaved were the highest in 1 Direct Current (DC) pulse group but there were no significant difference among all groups (p>0.05). The percentage of couplets successfully fused in 10 μsec group (68.3%) was significantly higher than 40 μsec group (37.5%, p<0.05). The percentage of reconstructed embryos cleaved was 64.7% at 2 h group, it was higher than 3 h group (26.0%, p<0.05). These results suggested that electrical fusion procedure of 2.4 kV cm-1 EFS, 1 DC pulse, 10 μsec and 2 h after NT manipulation were feasible to iSCNT of sika deer-bovine.
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How to cite this article:

Shiyong Wang, Junjun Zheng, Yuechun Yang, Zongyue Liu, Miao Yu, Chunyi Li and Fuhe Yang, 2014. Fusion Competence of Interspecies Nuclear Transfer Embryos Reconstructed from Pedicle Periosteum Cell of Sika Deer (Cervus nippon) and Oocyte of Bovine. Asian Journal of Animal Sciences, 8: 79-85.

DOI: 10.3923/ajas.2014.79.85

URL: https://scialert.net/abstract/?doi=ajas.2014.79.85

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