
	Asian Journal of Agricultural Research
	Year: 2014 \| Volume: 8 \| Issue: 5 \| Page No.: 259-268 DOI: 10.3923/ajar.2014.259.268

Control Activity of Potential Antifungal-Producing Burkholderia sp. in Suppressing Ganoderma boninense Growth in Oil Palm

Rika Fithri N. Buana, Aris Tri Wahyudi and Nurita Toruan-Mathius

Abstract: Basal Stem Rot (BSR) disease caused by G. boninense is one of the most serious diseases in oil palm. Many attempts have been done to prevent or reduce infection of this disease but they have not provided optimum results. Burkholderia sp. isolated from rhizosphere and root tissue of symptomless oil palm showed potentials in suppressing G. boninense growth in vitro. Five isolates of rhizosphere Burkholderia sp. and one endophyte Burkholderia sp. were used in antagonist test against G. boninense growth on PDA media. Antifungal biosynthesis related gene from Burkholderia sp. was confirmed by using PCR with pyrrolnitrin (prn), pyoluteorin (plt), phenazine (phz) and DAPG (phl) primers. Burkholderia sp. with the highest antagonist activity were applied to oil palm germinated seed for in vivo test. Endophyte Burkholderia B212 showed the highest antagonist activity against G. boninense growth in vitro with PIRG 34.38%. The B212 genome was yield an expected PCR product by using prn primers (790 bp). Sequence BLAST result showed the gene was 99% identical with B. cepacia partial prnD gene, strain ESR63. In vivo test of B212 showed that treatment of Burkholderia B212 on plant without G. boninense infection increased the height and biomass of the plant. However, B212 did not decrease the disease incidence and disease severity on G. boninense infected plant. In addition it decreased the plant height and biomass compare to control plant.

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How to cite this article:

Rika Fithri N. Buana, Aris Tri Wahyudi and Nurita Toruan-Mathius, 2014. Control Activity of Potential Antifungal-Producing Burkholderia sp. in Suppressing Ganoderma boninense Growth in Oil Palm. Asian Journal of Agricultural Research, 8: 259-268.

DOI: 10.3923/ajar.2014.259.268

URL: https://scialert.net/abstract/?doi=ajar.2014.259.268

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