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Asian Journal of Agricultural Research

Year: 2012 | Volume: 6 | Issue: 2 | Page No.: 60-72
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Authors

S.R.E. Abo-Hegazy

Noha F. El-Badawy

M.M. Mazen

H. Abd El-Menem

Keywords

Research Article

Evaluation of Some Faba Bean Genotypes against Chocolate Spot Disease Using cDNA Fragments of Chitinase Gene and Some Traditional Methods

S.R.E. Abo-Hegazy, Noha F. El-Badawy, M.M. Mazen and H. Abd El-Menem
Thirteen faba bean (Vicia faba L.) genotypes were assessed under greenhouse and field conditions in 2008/2009 and 2009/2010 seasons, using morphological and molecular characterization methods for resistance to chocolate spot diseases caused by Botrytis fabae A significant variation among the tested genotypes and their reaction to the disease was found under greenhouse condition. Sakha 1, 2 and Nubaria 1 were resistant, indicating by the lowest disease severity. Whereas, Giza 40, line 375 and Cairo 25 where susceptible. Other faba bean genotypes were moderately susceptible. RT-PCR analysis using chit 1 and 2 primers amplified fragment of chitinase gene (900 bp) in six faba bean genotype upon infection with the pathogen 48 h after inoculation. This fragment detected only in the resistance cultivars Sakha 1, 2 and Nubaria 1 and some moderate resistance genotype including Giza 3, line 24 H and line 36. At the same time no signal was detected in other infected genotypes or healthy ones. Field results of testing genotype against chocolate spot diseases often do not correspond completely with greenhouse results since the environment is less controlled and possibly natural infection could introduced unwanted pathogen(s) or other factors. The estimates of heritability in broad sense ranged from 0.90-0.99, high values of heritability were obtained for seed yield/plant (0.99), followed by plant dry weight, plant height, seeds pod-1 across the two seasons. However, No relationship was found between resistance of genotypes against B. fabae and their morphological characterizes under field conditions.
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How to cite this article

S.R.E. Abo-Hegazy, Noha F. El-Badawy, M.M. Mazen and H. Abd El-Menem, 2012. Evaluation of Some Faba Bean Genotypes against Chocolate Spot Disease Using cDNA Fragments of Chitinase Gene and Some Traditional Methods. Asian Journal of Agricultural Research, 6: 60-72.

DOI: 10.3923/ajar.2012.60.72

URL: https://scialert.net/abstract/?doi=ajar.2012.60.72

Comments

hamdy mohamed abo shedida Reply

This paper is useful l need all paper related this subject

Dr. Samir Abo Hegazy

Dear Dr. Hamdy
I am so sorry because i can't send it now for you, because i am not in Egypt now, may be after 2 monthes when i come back to Egypt, send you these papers

hamdy mohamed gamal eldeen aboshedida

dear dr samir/iam hamdy i requested from you the papers ralated this subject before and you tell me you will send them to me when you come back to egypt and i should be grateful to you if you send me these things

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