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Asian Journal of Agricultural Research

Year: 2007 | Volume: 1 | Issue: 2 | Page No.: 50-58
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Authors

Amel Kerkeni

Mejda Daami-Remadi

Neji Tarchoun

Mohamed Ben Khedher

Keywords

Research Article

In vitro and in vivo Suppression of Pythium ultimum the Causal Agent of the Cucumber Damping-Off by Some Compost Fungi

Amel Kerkeni, Mejda Daami-Remadi, Neji Tarchoun and Mohamed Ben Khedher
Seven fungi isolated from an animal manure compost are tested for their in vitro and in vivo antagonistic activity against Pythium ultimum, the causal agent of the cucumber damping-off. In vitro, dual culture experiments, observed after incubation at 25°C on PDA during 4 days, showed that six fungi inhibited by 18 to 61% the mycelial growth of P. ultimum, whereas one fungus showed no activity. Competition for media was the most remarkable mechanism of action noted on PDA. In vivo experiments, sterilized peat individually treated with Aspergillus sp., Trichoderma viride (strain 1 and strain 2), at a rate of 0.5 g L-1 and infested with P. ultimum was evaluated for its suppressive effect of the cucumber (Cucumis sativus L. Mornegui) damping-off. Results showed the tested fungi, incorporated to the culture substrates, decreased the in vivo development of P. ultimum, but at variable degrees, comparatively to the untreated control. Damping-off was lower for cucumber plants treated with T. viride’ (strain 2) than those treated with Aspergillus sp. and with T. viride (strain 1). Aspergillus sp. isolated from compost, which was inactive in vitro, suppressed the cucumber damping-off by 69.4%.
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