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Asian Journal of Applied Sciences
  Year: 2018 | Volume: 11 | Issue: 4 | Page No.: 192-198
DOI: 10.3923/ajaps.2018.192.198
Phytochemical and GC-MS Evaluation of Bioactive Principle of Vitis vinifera Peels
Uraku Anayo Joseph , Uraku Oluchi Helen, Nweke Friday Nwalo, Orji Obasi Uche, Igwenyi Ikechukwu Okorie, Edwin Nzubechukwu and Ezeani Nkiru Nwamaka

Abstract:
Background and Objectives: Vitis vinifera is small round or oval berry that feature semi-translucent flesh encased by a smooth skin. It is grown for its juice and the peels discarded as waste. This is done due to lack of awareness of the health benefits of its peels. This study was aimed to determine the phytochemicals and bioactive principle of V. vinifera peels. Materials and Methods: The peels of V. vinifera were obtained from the fruits and shade dried at room temperature. The dried peels were pulverized into fine powder. The phytochemicals and bioactive amalgams were done by method of AOAC and Gas Chromatography-Mass Spectrometry (GC-MS), respectively. Results: The results of phytochemical studies showed V. vinifera peels had high levels of alkaloids, phenol, tannins and oxalate with saponin low while vitamin contents had highest amounts of Vitamin A and B9, moderate amounts of C and E with trace amounts of B1, B2 and B5. Also, the results of proximate contents indicate that carbohydrate content was highest. Other findings are crude fibre, crude protein, fats, moisture and ash. Mineral analysis revealed the order K>Mg>Fe>Na>Ca in the Vitis vinifera. The mass spectrum of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. Seven bioactive compounds were identified in methanol peel extract by GC-MS analysis The result revealed that the peel had high peak areas (%) in the order of octadec-11-enoic acid, methyl ester, 9,12,15-octadecatrien-1-ol, hexadecanoic acid, Methyl 14-methyl pentadecanoate, stearic acid, methyl ester, 5-chloropyridin-2-ol and 8-hydroxyocta-2,4,6-trien-1-ylium. Conclusion: The findings therefore, suggest that there is an indication that V. vinifera peels contains chief phytochemicals and bioactive compounds that may be linked to its beneficial effects on health.
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How to cite this article:

Uraku Anayo Joseph, Uraku Oluchi Helen, Nweke Friday Nwalo, Orji Obasi Uche, Igwenyi Ikechukwu Okorie, Edwin Nzubechukwu and Ezeani Nkiru Nwamaka, 2018. Phytochemical and GC-MS Evaluation of Bioactive Principle of Vitis vinifera Peels. Asian Journal of Applied Sciences, 11: 192-198.

DOI: 10.3923/ajaps.2018.192.198

URL: https://scialert.net/abstract/?doi=ajaps.2018.192.198

 
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