M. B. Rokni
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran
A. Baghernejad
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran
M. Mohebali
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran
E. B. Kia
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran
ABSTRACT
The present study was targeted as examining sera obtaining from patients infected with Fasciola sp. by the Enzyme-linked Immunotransfer Blot (EITB) technique using the parasite`s Cysteine Proteinase (CE) antigen in order to evaluate the diagnostic potential of the assay. Altogether, a sort of sera including 80 cases of fasciolosis, 80 with other parasitosis other than fasciolosis and 30 normal control sera were enrolled in the trial. Hinge on the collected results, 78 fasciolosis serum samples recognized two antigenic polypeptides of 27 and 29 kDa. The sensitivity, specificity and positive and negative predictive values for CP antigen were 97.5, 98.8, 98.7 and 97.7%, respectively. Utterly, one case of cross-reaction was verified with a toxocariasis case. Concluding remark suggests that the 27 and 29 kDa bands in EITB test could be imperative in the immunodiagnosis of human fascioliasis.
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How to cite this article
M. B. Rokni, A. Baghernejad, M. Mohebali and E. B. Kia, 2006. Evaluation of Enzyme-linked Immunotransfer Blot for the Immunodiagnosis of Human Fascioliasis Using Cysteine Proteinase Antigen. Pakistan Journal of Biological Sciences, 9: 80-83.
DOI: 10.3923/pjbs.2006.80.83
URL: https://scialert.net/abstract/?doi=pjbs.2006.80.83
DOI: 10.3923/pjbs.2006.80.83
URL: https://scialert.net/abstract/?doi=pjbs.2006.80.83
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