Kairalla M.S. Khairalla
Molecualr Biology Laboratory, Department of Medicine Pharmacology and Toxicology
Badr E. Hago
Department of Surgery and Anaethesia, Faculty of Veterinary Medicine,
P.O. Box 32, Khartoum North, Sudan
Tigani Hassan
Intemational Center for Faith Research, Khartoum, Sudan
Ali A. Majid
National Council For Research, Ministry of Science and Technology, Khartoum, Sudan
El-Amin Dafalla
Department of Surgery and Anaethesia, Faculty of Veterinary Medicine,
P.O. Box 32, Khartoum North, Sudan
Abdul E. Karrar
Molecualr Biology Laboratory, Department of Medicine Pharmacology and Toxicology
Imadeldin E. Aradaib
Molecualr Biology Laboratory, Department of Medicine Pharmacology and Toxicology
ABSTRACT
Pork consumption is prohibited in some religions. Therefore, religious people are adament about importing processed food, which may contain or has been contaminated with pork or swine-derived products. In Sudan, no reliable assays exist for detecting the presence of pork in processed food. Currently, regulatory officials rely on a paper trail for this verification. To address the void in scientific regulatory monitoring, a means of a reliable, rapid, sensitive and specific method for detection of pork in processed food is urgently needed. The swine mitochondrial cytochrome-b (mtcyt-b) gene was used as a target DNA for PCR amplification. Using a pair of primers (PSL1 and PSR4), the mtcyt-b PCR resulted in amplification of a 525 base pair (bp) PCR product. The sensitivity of this mtcyt-b PCR was found to be 100 fg of DNA (equivalent to 1000 copies) as determined by DNA concentration and number of copies of mtcyt-b DNA, extracted from whole blood sample obtained from pigs. The mtcyt-b PCR assay provides a simple, rapid and reliable method for detection and identification of fresh, marinated or cocked pork in processed food produced for human consumption. In addition, this PCR assay should support future policies regarding import regulations for food industry.
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How to cite this article
Kairalla M.S. Khairalla, Badr E. Hago, Tigani Hassan, Ali A. Majid, El-Amin Dafalla, Abdul E. Karrar and Imadeldin E. Aradaib, 2005. Rapid Detection of Pork in Processed Food Using Polymerase Chain Reaction Amplification Technology: A Preliminary Report. Pakistan Journal of Biological Sciences, 8: 501-504.
DOI: 10.3923/pjbs.2005.501.504
URL: https://scialert.net/abstract/?doi=pjbs.2005.501.504
DOI: 10.3923/pjbs.2005.501.504
URL: https://scialert.net/abstract/?doi=pjbs.2005.501.504
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