Ozlem Sultan Aslanturk
Department of Biology, Faculty of Art and Science, Adnan Menderes University, 09010 Aydin, Turkey
Tulay Askin Celik
Department of Biology, Faculty of Art and Science, Adnan Menderes University, 09010 Aydin, Turkey
ABSTRACT
In this study, whether lycopene has preventive effect on chromosome aberrations or not was investigated. For this purpose, 2 different concentrations (0.02 and 0.03 M) of Ethyl Methane Sulfonate (EMS) which is known to induce chromosomal damage were treated on Allium cepa for 2 h. Afterwards, plant roots were treated with lycopene extracts in 4 different concentrations (1, 3, 5 and 10 μM) for 24 h. 0.02 and 0.03 M EMS were used as positive control and tap water was used as negative control. Root tip meristematic cells were observed under the microscope. Mitotic index (MI) and chromosomal aberrations (fragments, bridges, stickness, polar deviation) and micronucleus formation were evaluated and statistically analyzed. As a result; it was determined that depending on applied lycopene concentrations both in MI and chromosomal aberrations lycopene-caused some changes. It was seen that lycopene had preventive effect on chromosome aberrations particularly at 1 and 3 μM concentrations, but this effect decreased at 5 and 10 μM concentrations.
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How to cite this article
Ozlem Sultan Aslanturk and Tulay Askin Celik, 2005. Preventive Effect of Lycopene on Chromosome Aberrations in Allium cepa. Pakistan Journal of Biological Sciences, 8: 482-486.
DOI: 10.3923/pjbs.2005.482.486
URL: https://scialert.net/abstract/?doi=pjbs.2005.482.486
DOI: 10.3923/pjbs.2005.482.486
URL: https://scialert.net/abstract/?doi=pjbs.2005.482.486
REFERENCES
- Stahl, W. and H. Sies, 1996. Lycopene: A biologically important carotenoid for humans? Arch. Biochem. Biophys., 336: 1-9.
CrossRefPubMedDirect Link - Di Mascio, P., S. Kaiser and H. Sies, 1989. Lycopene as the most efficient biological carotenoid singlet oxygen quencher. Arch. Biochem. Biophys., 274: 532-538.
CrossRefDirect Link - Torbergsen, A.C. and A.R. Collins, 2000. Recovery of human lymphocytes from oxidative DNA damage: The apparent enhancement of DNA repair by carotenoids is probably simply an antioxidant effect. Eur. J. Nutr., 39: 80-85.
Direct Link - Fiskesjo, G., 1993. Allium test I: A 2-3 day plant test for toxicity assessment by measuring the mean root growth of onions (Allium cepa L.). Environ. Toxicol. Water Qual., 8: 461-470.
CrossRefDirect Link - Zhang, L.X., R.V. Cooney and J.S. Bertram, 1991. Carotenoids enhance gap junctional communication and inhibit lipid peroxidation in C3H/10T1/2 cells: Relationship to their cancer chemopreventive action. Carcinogenesis, 12: 2109-2114.
CrossRefDirect Link - Levy, J., E. Bosin, B. Feldman, Y. Giat, A. Miinster, M. Danilenko and Y. Sharoni, 1995. Lycopene is a more potent inhibitor of human cancer cell proliferation than either α-carotene or β-carotene. Nutr. Cancer., 24: 257-266.
CrossRefDirect Link - Nahum, A., K. Hirsch, M. Danilenko, C.K.V. Watts, O.W.I.J. Prall, J. Levy and Y. Sharoni, 2001. Lycopene inhibition on cell cycle progression in breast and endometrial cancer cells is associated with reduction in cyclin D levels and retention of p27Kip1 in the cyclin E-cdk2 complexes. Oncogene, 20: 3428-3436.