Z. A. Zakaria
School of Biotechnology and Life Sciences, Universiti Industri Selangor,
Jalan Zirkon A 7 I A, Seksyn 7,40000 Shah Alam, Selangor, Malaysia
M. R. Sulaiman
Department of Biomedical Sciences, Faculty of Medicine and Health Sciences,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
M. N. Somchit
Laboratory of Pharmaceutical and Neutriceutical, Institute of BioScience,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
A.M. Mat Jais
Department of Biomedical Sciences, Faculty of Medicine and Health Sciences,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
ABSTRACT
The onset and offset time of aqueous supernatant of haruan (Channa striatus) fillet (ASH) antinociception was evaluated using the abdominal constriction test. The ASH, extracted using chloroform:methanol (2:1; v:v) overnight and then evaporated to remove the residual solvent, was prepared in the concentration of 10, 25 and 100% and administered subcutaneously (SC) at four different set of time (0, 5, 30 and 60 min). At the end of the respective time, acetic acid (0.6%) was administered intraperitoneally (IP) and the number of abdominal constrictions was then calculated. ASH was found to exhibit a concentration-dependent antinociception. The onset time of antinociception was found to be between 0 to 5 min depending on the concentration of ASH used with the highest and lowest concentrations of ASH produced antinociception 0 and 5 min after their administration, respectively. This activity, which increased with increase in time of ASH administration, was found to reach the highest level after 30 min of ASH administration regardless of the concentration used. The offset time, on the other hand, does not depend on the concentration of ASH used. The three respective concentrations of ASH antinociception were found to diminish completely after 60 min of their administration. Interestingly, it was also found that ASH produced activity comparable to that of acetylsalicylic acid (ASA).
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How to cite this article
Z. A. Zakaria, M. R. Sulaiman, M. N. Somchit and A.M. Mat Jais, 2005. Evaluation of the Onset and Offset Time of Aqueous Supernatant of Haruan (Channa striatus) Fillet Antinociception Administered Subcutaneously in Mice. Pakistan Journal of Biological Sciences, 8: 466-469.
DOI: 10.3923/pjbs.2005.466.469
URL: https://scialert.net/abstract/?doi=pjbs.2005.466.469
DOI: 10.3923/pjbs.2005.466.469
URL: https://scialert.net/abstract/?doi=pjbs.2005.466.469
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