R. Ben Ammar
Laboratoire de Biologie Cellulaire et Mol6culaire, Facult6 de M6decine Dentaire de Monastir,
Rue Avicenne 5000 Monastir, Tunisia
S. Kilani
Laboratoire de Biologie Cellulaire et Mol6culaire, Facult6 de M6decine Dentaire de Monastir,
Rue Avicenne 5000 Monastir, Tunisia
A. Abdelwahed
Laboratoire de Biologie Cellulaire et Mol6culaire, Facult6 de M6decine Dentaire de Monastir,
Rue Avicenne 5000 Monastir, Tunisia
N. Hayder
Laboratoire de Biologie Cellulaire et Mol6culaire, Facult6 de M6decine Dentaire de Monastir,
Rue Avicenne 5000 Monastir, Tunisia
A. Mahmoud
Laboratoire de Pharmacognosie, Facult6 de Pharmacie de Monastir, Rue Avicenne 5000 Monastir, Tunisia
J. Chibani
Laboratoire de Biochimie et de Biologie Mol6culaire,
Facult6 de Pharmacie de Monastir, Rue Avicenne 5000 Monastir, Tunisia
L. Chekir-Ghedira
Laboratoire de Biologie Cellulaire et Mol6culaire, Facult6 de M6decine Dentaire de Monastir,
Rue Avicenne 5000 Monastir, Tunisia
K. Ghedira
Laboratoire de Pharmacognosie, Facult6 de Pharmacie de Monastir, Rue Avicenne 5000 Monastir, Tunisia
ABSTRACT
Nine Rhamnus alaternus extracts were tested for both mutagenic and antimutagenic effects using the Salmonella microsome assay with the two tester strains Salmonella typhimurium TA98 and TA100. None of these extracts was mutagenic. The antimutagenicity of the same extracts was also tested against the S9-dependent mutagen, Aflatoxin B1 (AFLB1) (10 μg/assay). Total Oligomers Flavonoids (TOF), ethyl acetate and methanol extracts showed the strongest inhibition of the mutagenicity induced by AFLB1 in both TA98 and TA100 assay system. In addition to antimutagenic activity, the TOF and the ethyl acetate extracts showed an important free radical scavenging activity towards the 1,1-diphenyl-2-picrylhydrazyd (DPPH) radical. These results suggest the future utilization of these extracts as additives in chemoprevention studies.
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How to cite this article
R. Ben Ammar, S. Kilani, A. Abdelwahed, N. Hayder, A. Mahmoud, J. Chibani, L. Chekir-Ghedira and K. Ghedira, 2005. In vitro Mutagenicity, Antimutagenicity and Free Radical Scavenging Activities of Rhamnus alaternus L. (Rhamnaceae) Extracts. Pakistan Journal of Biological Sciences, 8: 439-445.
DOI: 10.3923/pjbs.2005.439.445
URL: https://scialert.net/abstract/?doi=pjbs.2005.439.445
DOI: 10.3923/pjbs.2005.439.445
URL: https://scialert.net/abstract/?doi=pjbs.2005.439.445
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