S. Centeno
Microbiology, School of Veterinary Science, Universitat Autonoma de Barcelona, 08193 Barcelona, Spain
C. Adelantado
Microbiology, School of Veterinary Science, Universitat Autonoma de Barcelona, 08193 Barcelona, Spain
M. A. Calvo
Microbiology, School of Veterinary Science, Universitat Autonoma de Barcelona, 08193 Barcelona, Spain
ABSTRACT
Competition between micro-organisms and the production of inhibitor substances by some of these micro-organisms is frequent in substrata with varied, complex microbiota, such as cork. The main aim of this study was to determine the inhibitor activity of filamentous fungi, yeasts and bacteria isolated from wine bottle corks. The inhibitor activity of isolated micro-organisms was determined by placing disks of pure culture in Petri dishes containing 2% malt extract agar for fungi and trypticase soy agar for bacteria. These were surface-seeded beforehand with the test micro-organism, the dishes are incubated in conditions suitable for the growth of the surface-seeded micro-organisms and finally the inhibitor plaques produced are measured. The results indicate that the micro-organisms with the greatest inhibitor activity are Penicillium citrinum and Trichoderma viride.
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How to cite this article
S. Centeno, C. Adelantado and M. A. Calvo, 2004. Inhibitory Activity of Micro-organisms Isolated from Wine Bottle Corks. Pakistan Journal of Biological Sciences, 7: 2198-2201.
DOI: 10.3923/pjbs.2004.2198.2201
URL: https://scialert.net/abstract/?doi=pjbs.2004.2198.2201
DOI: 10.3923/pjbs.2004.2198.2201
URL: https://scialert.net/abstract/?doi=pjbs.2004.2198.2201
REFERENCES
- Danesh, P., F.M. Caldas, J.J. Figuereido and M.V. San Romao, 1997. Mycobiota in Portuguese normal and green cork throughout the manufacturing process of stoppers. J. Applied Microbiol., 82: 689-694.
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