A. M. Al-swailem
King Abdulaziz City for Science and Technology
A. M. Shibl
Natural Resources and Environment Institute, Saudi Arabia
O. H. Shair
King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 11442,
A. A. Kadry
Department of Pharmaceutics, Faculty of Pharmacy, King Saud University
ABSTRACT
In the present work studies of 150 local Streptococcus pneumoniae isolates from different hospitals in Riyadh, by polymerase chain reaction ( PCR) techniques revealed 18 clones to be ampicillin resistant. The genetic relatedness of 18 (12%) of the 150 clones ampicillin resistant isolates to 9 wild type ampicillin resistant clones was determined through Random Amplified Polymorphic DNA (RAPD). Similarity matrix and phylogenetic tree of the RAPD types showed that the ampicillin resistant wild type were not closely related to ampicillin resistant local isolates. The cluster analysis by unweighed-paired group of arithmetic mean (UPGMA) also does not indicated genetic proximity between wild type and the local isolates. The present study shows that there are no new clones identified. The local clones were not very closely genetically related to each other. The local sp. 29 and local sp. 36 were the two most related strains among the 18 strains with value (0.32) in the similarity range. While different internationally widespread ampicillin resistant clones exist in the Kingdom, in this study there is no common clone spread in the area was found. This study will help in the development of awareness about the hazards caused by the resistant clones.
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How to cite this article
A. M. Al-swailem, A. M. Shibl, O. H. Shair and A. A. Kadry, 2004. Genetic Relatedness of Ampicillin Resistant Streptococcus pneumoniae Clones from Saudi Arabia to Wild Strains. Pakistan Journal of Biological Sciences, 7: 1346-1349.
DOI: 10.3923/pjbs.2004.1346.1349
URL: https://scialert.net/abstract/?doi=pjbs.2004.1346.1349
DOI: 10.3923/pjbs.2004.1346.1349
URL: https://scialert.net/abstract/?doi=pjbs.2004.1346.1349
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