M. M. Rahman
Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh
A.B.S.M. Osman Gani
Department of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh
M. A. Choudhury
Department of Pharmacy, University of Science and Technology, Chittagong (USTC), Bangladesh
M. H. Rahman
Department of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh
ABSTRACT
The binding of dexamethasone phosphate (DP) and testosterone phenyl propionate, (TPP) two semisynthetic steroids, to bovine serum albumin (BSA) was studied by equilibrium dialysis method at 25°C and pH 7.4 with a view to have an insight into the competitive binding characteristics of these two drugs, when bound to BSA simultaneously. There was increase in free concentration of DP due to addition of TPP and vice versa during concurrent administration of these two drugs, thereby causing reduced binding of these two drugs to BSA. However, the free fraction was not increased up to a level as it was expected from direct competitive displacement. In absence of the site I specific probe (warfarin sodium), DP after being displaced by TPP or vice versa from its high affinity binding site (site II) rebound to its low affinity binding site (site I) on BSA. However, when the site I was sufficiently blocked by warfarin, the increment in the free concentration of the displaced drug was more prominent. This form of modified displacement has been referred to as site-to-site displacement.
PDF References Citation
How to cite this article
M. M. Rahman, A.B.S.M. Osman Gani, M. A. Choudhury and M. H. Rahman, 2001. Binding of Dexamethasone Phosphate and Testosterone Phenyl Propionate to
Bovine Serum Albumin: Drug?drug Interaction at the Binding Site. Pakistan Journal of Biological Sciences, 4: 572-574.
DOI: 10.3923/pjbs.2001.572.574
URL: https://scialert.net/abstract/?doi=pjbs.2001.572.574
DOI: 10.3923/pjbs.2001.572.574
URL: https://scialert.net/abstract/?doi=pjbs.2001.572.574
REFERENCES
- Kober, A. and I. Sjoholm, 1980. The binding sites on human serum albumin for some nonsteroidal antiinflammatory drugs. Mol. Pharmacol., 18: 421-426.
Direct Link - Kragh-Hansen, U., 1981. Molecular aspects of ligand binding to serum albumin. Pharmacol. Rev., 33: 17-53.
PubMed - Sudlow, G., D.J. Birkett and D.N. Wade, 1976. Further characterization of specific drug binding sites on human serum albumin. Mol. Pharmacol., 12: 1052-1061.
Direct Link