Samad Akbarzadeh
Department of Biochemistry, Bushehr University of Medical Sciences, Bushehr, Iran
Mohsen Ani
Department of Clinical Biochemistry, Isfahan University of Medical Sciences, Isfahan, Iran
Ali A. Moshtaghie
Department of Clinical Biochemistry, Isfahan University of Medical Sciences, Isfahan, Iran
Ali Movahed
Department of Clinical Biochemistry, Isfahan University of Medical Sciences, Isfahan, Iran
ABSTRACT
The effects of selenium as an antioxidant on erythrocyte Sodium-Lithium Countertransport (SLC) activity and plasma parameters have not already been studied in full detail. In the present study, the relationship between selenium and SLC activity, plasma parameters (lipids, lipoproteins, sodium, potassium, urea and creatinine) was investigated. Male rabbits weighed 1350±50 g were divided in to four groups (5 in each). For in vivo studies selenium dioxide (SeO2) was administered intraperitoneally on alternate days for 2 weeks as acute dose (250 μg kg-1 body weight) and for 7 weeks as chronic dose (125 μg kg-1 body weight). The control groups for each doses received deionized water at the same time. The results showed that the acute dose of selenium decreased SLC activity and Vmax/Km, but the Km of the transporter was increased. Also this metal decreased plasma concentrations of cholesterol, triglyceride and VLDL. All these changes may be helpful to the health. The chronic dose of the metal increased SLC activity, Vmax/Km and Vmax, but it decreased Km of the transporter. This dose of selenium increased plasma concentrations of cholesterol, triglyceride, VLDL, LDL, sodium and decreased the potassium level. In vitro studies showed that, the metal at low concentration (1 μM) can lower SLC activity and then it is beneficial to the health, but at high concentration (50 and 100 μM) increases the SLC activity and may cause a serious problem to the health. It is concluded that selenium at high concentration and with chronic dose increases the SLC activity and plasma lipids. But at low concentration and acute dose will have a beneficial effect to the health.
PDF References
How to cite this article
Samad Akbarzadeh, Mohsen Ani, Ali A. Moshtaghie and Ali Movahed, 2008. Changes in Erythrocyte Sodium-Lithium Countertransport and Plasma Parameters Following Selenium Treatment. Journal of Biological Sciences, 8: 166-170.
DOI: 10.3923/jbs.2008.166.170
URL: https://scialert.net/abstract/?doi=jbs.2008.166.170
DOI: 10.3923/jbs.2008.166.170
URL: https://scialert.net/abstract/?doi=jbs.2008.166.170
REFERENCES
- Ani, M., A.A. Moshtaghie and S. Akbarzadeh, 2006. Changes in sodium-lithium countertransport activity following aluminium treatment. Pak. J. Biol. Sci., 9: 1950-1954.
CrossRefDirect Link - Ani, M., A.A. Moshtaghie and S. Akbarzadeh, 2007. The effect of lead on red blood cell sodium-lithium countertransport and induction of hypertension. J. Cell Tiss. Res., 7: 981-985.
Direct Link - Batuman, V., A. Dreisbach, E. Chun and M. Naumoff, 1989. Lead increases red cell sodium-lithium countertransport. Am. J. Kidney Dis., 14: 200-203.
CrossRefDirect Link - Canessa, M., N. Adragna, H.S. Solomon, T.M. Connolly and D.C.Tosteson, 1980. Increased sodium-lithium countertransport in red cells of patients with essential hypertension. New Engl. J. Med., 302: 772-776.
Direct Link - Duhm, J. and B.F. Becker, 1979. Studies on lithium transport across the red cell membrane. V. on the nature of the Na+-dependent Li+ countertransport system of mammalian erythrocytes. J. Membr. Biol., 51: 263-286.
CrossRefDirect Link - Gruska, S., I. Jendral, R. Rettig and G. Kraatz, 2003. Sodium/lithium countertransport and intracellular calcium concentration in patient with essential hypertension and coronary heart disease. Clin. Sci., 104: 323-327.
Direct Link - Hardman, T.C. and A.F. Lant, 1996. Controversies surrounding erythrocyte sodium-lithium countertransport. J. Hypertens., 14: 695-703.
Direct Link - Holben, D.H. and A.M. Smith, 1999. The diverse role of selenium within selenoproteins: A review. J. Am. Dietetic Assoc., 99: 836-843.
CrossRefDirect Link - Hsu, P.C. and Y.L. Guo, 2002. Antioxidant nutrients and lead toxicity. Toxicology, 180: 33-44.
CrossRefDirect Link - Jennings, M.L., M. Adams-Lackey and K.W. Cook, 1985. Absence of significant sodium-hydrogen exchange by rabbit erythrocyte sodium-lithium countertransporter. Am. J. Physiol.-Cell Physiol., 249: C63-C68.
Direct Link - Lang-Lazdunski, L., C. Heurteaux, H. Dupont, C. Widmann and M. Lazdunski, 2000. Prevention of ischemic spinal cord injury: Comparative effects of magnesium sulfate and riluzole. J. Vascular Surg., 32: 179-189.
Direct Link - Laurenzi, M., M. Cirillo, W. Panarelli, M. Revisan and R. Stamler et al., 1997. . Baseline sodium-lithium countertransport and 6-year incidence of hypertension. The gubbio population study. Circulation, 95: 581-587.
CrossRefDirect Link - Liu, W.M., Z.G. Zhu and H.X. Leng, 2004. Analysis of the contents of K, Na, Ca, Mg, Zn, Cu, Fe and Mn in serum of middle and old-aged hypertension patients. Guang Pu Xue Yu Guang Pu Fen Xi, 24: 360-362.
Direct Link - Mead, P., R. Wilkinson and T.H. Thomas, 1999. Thiol protein defect in sodium-lithium countertransport in subset of essential hypertension. Hypertension, 34: 1275-1280.
CrossRefDirect Link - Mead, P.A., R. Wilkinson and T.H. Thomas, 2001. Na/Li countertransport abnormalities in type 1 diabetes with and without nephropathy are familial. Diabetes Care, 24: 598-601.
Direct Link - Mu, J., Z. Liu, D. Yang, Y. Liang and Z. Wang, 2004. Sodium-lithium countertransport and the risk of children: A 10-year prospective study in China. Am. J. Hypertens., 17: S99-S99.
Direct Link - Nishiyama, S., K. Nakamura and Y. Konishi, 1987. Effect of selenium on blood pressure, urinary sodium excretion and plasma aldosterone in cadmium-treated male rats. Arch. Toxicol., 59: 365-370.
CrossRefDirect Link - Patrick, L., 2004. Selenium biochemistry and cancer: A review of the literature. Alter. Med. Rev., 9: 239-258.
Direct Link - Ragone, E., P. Strazzullo, A. Siani, R. Iacone and L. Russo et al., 1998. Ethnic differences in red blood cell sodium/lithium countertransport and metabolic correlates of hypertension. Am. J. Hypertens., 11: 935-941.
CrossRefDirect Link - Rayman, M.P., 2000. The importance of selenium to human health. Lancet, 356: 233-241.
CrossRefPubMedDirect Link - Rutherford, P.A., T.H. Thomas and R. Wilkinson, 1997. Na-Li countertransport kinetics in the relatives of hypertensive patients with abnormal Na-Li counter transport activity. Biochem. Mol. Med., 62: 106-112.
CrossRefDirect Link - Schork, N.J., J.P. Gardner, L.I. Zhang, D. Fallin, B. Thiel, H. Jakubowski and A. Aviv, 2002. Genomic association/linkage of sodium lithium countertransport in CEPH pedigrees. Hypertension, 40: 619-628.
Direct Link - Schweizer, U., A.U. Brauer, J. Kohrle, R. Nitsch and N.E. Savaskan, 2004. Selenium and brain function: A poorly recognized liaison. Brain Res. Rev., 45: 164-178.
Direct Link - Sieja, K. and M. Talerczyk, 2004. Selenium as an element in the treatment of ovarian cancer in women receiving chemotherapy. Gynecol. Oncol., 93: 320-327.
Direct Link - Tapiero, H., D.M. Townsend and K.D. Tew, 2003. The antioxidant role of selenium and seleno-compounds. Biomed. Pharmacother., 57: 134-144.
Direct Link - Thomas, T.H., I.C. West and R. Wilkinson, 1995. Modification of erythrocyte Na+/Li+countertransport kinetics by two types of thiol group. Biochim. Biophys. Acta-Biomembranes, 1235: 317-322.
CrossRefDirect Link - Thomas, T.H., P.A. Rutherford, I.C. West and R. Wilkinson, 1995. Sulphydryl group control of sodium-lithium countertransport kinetics: A membrane protein control abnormality in essential hypertension. Eur. J. Clin. Invest., 25: 235-240.
CrossRefDirect Link - Thomson, C.D., 2004. Assessment of requirements for selenium and adequacy of selenium status: A review. Eur. J. Clin. Nutr., 58: 391-402.
Direct Link - Vareesangthip, K., V. Thongtang, P. Hanlakorn, P. Pidetcha, L. Suwannaton and L. Ong-Aj-Yooth, 2001. Erythrocyte sodium lithium countertransport in heart transplantation. Transplant. Proc., 33: 3561-3563.
Direct Link - Vareesangthip, K., P. Hanlakorn, L. Suwannaton, P. Pidetcha and L. Ong-Aj-Yooth, 2004. Abnormal kinetics of erythrocyte sodium lithium counter transport in renal transplant recipients. Transplant. Proc., 36: 1367-1371.
Direct Link - Vaziri, N.D. and D.A. Sica, 2004. Lead-induced hypertension: Role of oxidative stress. Curr. Hypertens. Rep., 6: 314-320.
Direct Link - West, I.C., P.A. Rutherford and T.H. Thomas, 1998. Sodiumālithium countertransport: Physiology and function. J. Hypertens., 16: 3-13.
Direct Link - Zerbini, G., G. Ceolotto, C. Gaboury, L. Mos and A.C. Pessina et al., 1995. Sodium-lithium countertransport has low affinity for sodium in hyperinsulinemic hypertensive subjects. Hypertension, 25: 986-993.
CrossRefDirect Link