M. M. Ahasan
Animal Health Research Division, Bangladesh Livestock Research institute, Savar, Dhaka-1341, Bangladesh
K.M. Hossain
Biotechnology Discipline, Khulna University, Khulna-9208, Bangladesh,
M.R. Islam
Animal Health Research Division, Bangladesh Livestock Research institute, Savar, Dhaka-1341, Bangladesh
ABSTRACT
This experiment was conducted to study the adaptation of IBDV on vero cell line. Suspected IBDV isolates were collected from the bursa of dead chicken of a particular flock. For adaptation, the anchorage-dependant monolayer of vero cell was first subcultured to form semi-confluent monolayer in eagle`s minimum essential medium (EMEM) with 5% fetal calf serum. This semi-confluent monolayer was then infected by local field IBDV isolates. The passage 1 (P1) viruses were harvested and used for the next passage. In this way, the viruses were given three serial passages on vero cell line where characteristic cytopathic effects (CPEs) were observed. During the first passage, no CPEs were found. The infectivity of IBDV on vero cells was observed by changes in the characteristics of vero cell monolayer. During the third passage, clear and consistent CPEs were observed. Vero cell monolayer was changed to form rounding cells. The P3 adapted viruses were confirmed to be IBDV by agar gel precipitation test. The P3 IBDV virus became well adapted to vero cell line.
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How to cite this article
M. M. Ahasan, K.M. Hossain and M.R. Islam, 2002. Adaptation of Infectious Bursal Disease Virus (IBDV) on Vero Cell Line. Journal of Biological Sciences, 2: 633-635.
DOI: 10.3923/jbs.2002.633.635
URL: https://scialert.net/abstract/?doi=jbs.2002.633.635
DOI: 10.3923/jbs.2002.633.635
URL: https://scialert.net/abstract/?doi=jbs.2002.633.635
REFERENCES
- Van den Berg, T.P., 2000. Acute infectious bursal disease in poultry: A review. Avian Pathol., 29: 175-194.
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