Hala A. Farrag
Department of Drug Radiation Research, National Center for Radiation Research and Technology, P.O. Box 29, Nasr City, Cairo, Egypt
ABSTRACT
Adhesion of bacteria differing in cell surface hydrophobicity, growth and viability of single isolates of Klebsiella pneumonia, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus epidermidis isolated from catheter associated infections to catheter made of polyurethane, hydrophilic polyurethane, teflon, vialon and siliconised latex was evaluated before and after In vitro exposure to test dose of 25 Gy of gamma radiation. The cell surface hydrophobicity of the tested strains was assessed by hexadecane method. Hydrophobic strains adhered more efficiently to the catheter surface than hydrophilic strains. Low number of the hydrophilic Staphylococcus epidermidis cells can adhere to the polyurethane catheter surface. Irradiation cause a change in the cell surface hydrophobicity of the tested strains. Colonization of polyurethane and hydrophilic polyurethane in phosphate buffered saline (PBS) to non-irradiated and irradiated Klebsiella pneumonia and Escherichia coli was followed by scanning electron microscopy (SEM). Regular sampling of specimens at 1h , 3 h, 8 h, 12h,24 h and 48h. demonstrated colonization and adhesion progression with an increase in the exposure interval of the non irradiated cells to the polyurethane catheter surface followed by cell proliferation, possible break down of catheter components and production of a slimy material covering the bacterial colonies. Adherence and colonization of the irradiated cells showed reduction in cells number along the incubation time with abnormalities in the cells shape and size. While, the adherence of the non-irradiated and irradiated cells to the hydrophilic polyurethane surface showed marked decrease in number of cells with abnormalities in the cells shape and size. Bacterial adherence and colonization of catheters in distilled water were the same as in PBS and slightly delayed in the microcolony formation. Bacterial viability and growth was evaluated in eluates obtained from incubation of segments of each catheter in buffer for 24h. Non of the eluates increase the viability and growth of Staphylococcus epidermidis along the incubation time. However, all of them, significantly increased the growth of non-irradiated (Klebsiella pneumonia, Pseudomonas aeruginosa and Escherichia coli ) with the exception of the eluate from siliconised latex, in which the inoculum count was reduced for E. coli. The initial growth in different catheter eluates was higher for non-irradiated strains than irradiated ones with all biomaterials tested. So, bacterial adherence to catheter may depend in part on the nature of the biomaterial and that substances eluted from the catheters may affect the viability and growth of different microorganisms . The non irradiated strains can grow in catheter eluates and colonies catheter surface better than the irradiated strains. Although, The irradiation change the hydrophobicity of the tested strains as well as reduce the number of cells with abnormalities in shape and size , the irradiated strains also persist on these biomaterials. The implications of these findings may be important in the pathogenesis of foreign body infections and utilization of new biomaterials to prevent bacterial adherence and colonization in immuno-compromised patients.
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How to cite this article
Hala A. Farrag, 2001. In vitro Effect of Low Radiation Doses on Some Pathogenic Bacteria. Its Relation
to Surface Hydrophobicity, Adherence and Viability on Plastic Catheter Materials. Journal of Biological Sciences, 1: 966-975.
DOI: 10.3923/jbs.2001.966.975
URL: https://scialert.net/abstract/?doi=jbs.2001.966.975
DOI: 10.3923/jbs.2001.966.975
URL: https://scialert.net/abstract/?doi=jbs.2001.966.975
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