Hamid Rashid
Agricultural Biotechnology Institute, National Agricultural Research Centre, Park Road, Islamabad
Syed Yassir Abbas Bokhari
Department of Biological Science, University of Arid Agriculture, Rawalpindi, Pakistan
Azra Quraishi
Agricultural Biotechnology Institute, National Agricultural Research Centre, Park Road, Islamabad
ABSTRACT
A protocol has been developed for callus induction, regeneration and hygromycin selection of rice (Oryza sativa L. cv. Super Basmati). Mature seeds of rice were used as starting material for callus induction and cultured on Murashige & Skoog medium (MS) and N6 medium having different levels and combinations of 2,4-Dicholro Phenoxy Acetic Acid (2,4-D). 2,4-D @ 2 mg/l gave good response to callus induction. For regeneration, calli were cultured on MS medium having different concentrations of Naphthalene Acetic Acid (NAA) and Benzyl Amino Purine (BAP). Medium containing NAA @ 1mg/l with BAP 5 mg/l showed best results with regeneration frequency of 45.3%. Hygromycin at the concentration of 50 mg/l proved to be lethal for secutellum-darived calli of Super Basmati.
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How to cite this article
Hamid Rashid, Syed Yassir Abbas Bokhari and Azra Quraishi, 2001. Callus Induction, Regeneration and Hygromycin Selection of Rice (Super Basmati). Journal of Biological Sciences, 1: 1145-1146.
DOI: 10.3923/jbs.2001.1145.1146
URL: https://scialert.net/abstract/?doi=jbs.2001.1145.1146
DOI: 10.3923/jbs.2001.1145.1146
URL: https://scialert.net/abstract/?doi=jbs.2001.1145.1146
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