Kuhlmann .
Department of Cardiology and Angiology, Justus-Liebig-University of Giessen, Germany
Christoph Rudiger Wolfram
Department of Cardiology and Angiology, Justus-Liebig-University of Giessen, Germany
Gundlack Jurgen Ralf
Department of Internal Medicine, Hospital Schw�bisch-Hall, Germany
Heidt Martin
Department of Cardiology and Angiology, Justus-Liebig-University of Giessen, Germany
Tillmanns Harald
Department of Cardiology and Angiology, Justus-Liebig-University of Giessen, Germany
Waldecker Bernd
Department of Cardiology and Angiology, Justus-Liebig-University of Giessen, Germany
Wiecha Johannes
Department of Internal Medicine, Hospital Bad Orb, Germany
ABSTRACT
The present study investigated whether Plantlet Derived Growth Factor (PDGF) modulates Ca2+-activated K+ channels with large conductance and examined possible signaling mechanisms. BKCa activity was analyzed using the patch-clamp technique in cultured human endothelial cells derived from umbilical cord veins (HUVEC). Continuous perfusion of HUVEC with 30 ng mL -1 PDGF caused a significant increase of BKCa open-state probability (n=17; p<0.05) after 3 min. This BKCa activation was inhibited by preincubating the cells with pertussistoxin (100 ng mL -1). Despite the fact that the BKCa was activated by a release of Ca2+ from internal stores, this study could not observe a reduction of the open-state probability, if the internal release of Ca2+ was blocked using procaine (n=13; p=n.s.). In conclusion PDGF activates BKCa involving a G-protein dependent mechanism. This BKCa activation may play an important role in PDGF mediated effects on human endothelial cells.
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How to cite this article
Kuhlmann ., Christoph Rudiger Wolfram, Gundlack Jurgen Ralf, Heidt Martin, Tillmanns Harald, Waldecker Bernd and Wiecha Johannes, 2005. PDGF-BB Induced Modulation of Ca2+-Activated K+ Channels Involves a G-Protein Dependent Mechanism in Cultured Human Endothelial Cells. International Journal of Pharmacology, 1: 33-37.
DOI: 10.3923/ijp.2005.33.37
URL: https://scialert.net/abstract/?doi=ijp.2005.33.37
DOI: 10.3923/ijp.2005.33.37
URL: https://scialert.net/abstract/?doi=ijp.2005.33.37
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