Research Journal of Toxins1819-35602151-7258Academic Journals Inc.10.3923/rjt.2016.8.14Diodon hystrix Puffer Fish from South East Indian Coast]]>RaviLokesh ManuAdwaid ChocalingumRiven MentaVignesh KumarVishakha KhannaGopiesh 1201681In the present study, tetrodotoxin (TTX) extracted from eyes, skin, liver, intestine and gonads of Diodon hystrix were evaluated for toxicity study. The COX1 gene sequencing revealed 99% similarity to D. hystrix . This identification of D. hystrix from Chennai coastal region is the first molecular report providing new data about their geographical distribution. The GC-MS analysis revealed the presence of TTX toxin
in all the crude extracts of the fish except eye. Human lymphocyte chromosome aberration assay was tested using crude extracts
containing TTX (0.5 mg mL1) which caused spindle fibre aberration and comet assay showed lack of DNA damage. In support of genotoxicity, all the crude extracts containing TTX was tested on zebra fish, using 1 mL of 5 mg mL1 concentration stock solution. The results suggest that extracts from skin, gonads and liver of D. hystrix caused 100% mortality within 72 h. Based on these observations it is suggested that D. hystrix from chennai coastal region as food is a potential risk to humans.]]>Alcock, J.,20106234236Aligizaki, K., P. Katikou, A. Milandri and J. Diogene,201157390399Altschul, S.F., W. Gish, W. Miller, E.W. Myers and D.J. Lipman,1990215403410Arvanitoyannis, I.S., K.V. Kotsanopoulos and A. Papadopoulou,20145414731528Bane, V., M. Lehane, M. Dikshit, A. O'Riordan and A. Furey20146693755Bricelj, V.M., L. Connell, K. Konoki, S.P. MacQuarrie, T. Scheuer, W.A. Catterall and V.L. Trainer,2005434763767Campbell, K.P. Barnes, S.A. Haughey, C. Higgins, K. Kawatsu, V. Vasconcelos and C.T. Elliott,201340577537763Frame, K.K. and W.S. Hu,199035469476Geffeney, S., E.D. Brodie Jr., P.C. Rubben and E.D. Brodie III,200229713361339Hafeezullah, M.,1988Tetrochetus looss, 1912 (Digenea: Hemiuroidea: Accacoeliidae) occurring in Indian marine fishes.]]>82349361Kessler, N., M. Aymard and S. Bertrand,199329203207Khora, S.S., K.K. Panda and B.B. Panda,1997Allium cepa L.]]>12265269Kreuzer, M.P., M. Pravda, C.K. O'Sullivan and G.G. Guilbault,20024012671274Malpezzi, E.L., J.C. de Freitas and F.T. Rantin,1997355765Mohd Nor, A.A. and M.N. Wan Norhana,2013Xenopterus naritus) eggs from Satok market, Kuching, Sarawak.]]>2029632966Mohid Nor, A.A., M. Samsur and M. Othman,20144310031011Nagashima, Y., T. Matsumoto, K. Kadoyama, S. Ishizaki and S. Taniyama et al.,2012Lagocephalus inermis and the toxicity of L. inermis caught off the Kyushu coast, Japan.]]>538590Nishiguchi, M.K., P. Doukakis, M. Egan, D. Kizirian and A. Phillips et al.,20022002pp: 249-281pp: 249-281Noguchi, T., O. Arakawa and T. Takatani,20061145152Folmer, O., M. Black, W. Hoeh, R. Lutz and R. Vrijenhoek,1994c oxidase subunit I from diverse metazoan invertebrates.]]>3294299Puech, B., B. Batsalle, P. Roget, J. Turquet and J.P. Quod et al.,2014Lagocephalus sceleratus (Pufferfish).]]>1077984Radhakrishnan, S. and N.B. Nair,1981Tetrochetus coryphaenae (Digenea: Accacoelidae) infection of Diodon hystrix (Pisces: Diodontidae).]]>B474752Sneath, P.H.A. and R.R. Sokal,19731st Edn.,Pages: 573Pages: 573Tamura, K., J. Dudley, M. Nei and S. Kumar,20072415961599Thompson, J.D., D.G. Higgins and T.J. Gibson,19942246734680Tian, L., J. Cheng, X. Chen, S.H. Cheng and Y.L. Mak et al.,2014Oryzias melastigma) embryos.]]>771625Wang, R., A. Huang, L. Liu, S. Xiang and X. Li (et al).,2014832234Wu, Y.J., Y.J. Cheng, H.C. Jen, C.H. Pan, T.C. Lin, S.J. Lin and D.F. Hwang,201174789795Yakes, B.J., K.M. Kanyuck and S.L. DeGrasse,20148692519255Yong, Y.S., L.S. Quek, E.K. Lim and A. Ngo,20132013Yotsu-Yamashita, M., A. Sugimoto, A. Takai and T. Yasumoto,199928916881696Felsenstein, J.,198539783791Saito, H., R. Tabeta and K. Ogawa,19872024242430