Research Journal of Phytochemistry1819-34712151-6081Science International10.3923/rjphyto.2017.74.84NadjiSaid AbdaouiAbderahmen OuerdaneHanane AzzouzMohamed AbtrounRania ReggabMohamed AlamirBachra 22017112Background: Oleander is a widespread evergreen shrub belonging to the family of Apocynaceae, it can grow to reach 20 feet high, the plant contains glycosides in all parts with different levels. Intoxications usually occur after the ingestion of oleanders material and are featured by a scope of clinical symptoms ranging from digestive to cardiac signs and causing in some cases death. Objective: The main toxic is oleandrin, its levels vary considerably, the study aims to evaluate the levels of oleandrin in samples of oleanders (Nerium oleander L.) material collected from different regions of Algeria and the study of some factors that may influence it. Materials and Methods: The plant material mainly leaves but also flowers and branches were collected across 25 sites throughout Algeria, oleandrin, extracted by liquid-liquid extraction and analyzed by high performance liquid chromatography coupled to a diode array detector (HPLC-DAD), the samples were divided into groups and statistical tests (parametric and non-parametric) were performed. Results: The results show that leaves contain the paramount levels varying from 0.022-0.549%. Whereas, flowers and branches oleandrin levels range respectively from 0.005-0.025 and 0.004-0.062%. Conclusion: Significant variability was noted, related to the plant "Variety", height and habitat and certain conditions such as exposure to light or the nature of the soil.]]>Knight, A.P. and R.G. Walter,2001Pages: 367Pages: 367Odenwald, N.G. and J.R. Turner,20064th Edn.,Pages: 720Pages: 720Portis, E., C. Comino, A. Lenzi, P. Lombardi, R. Tesi and S. Lanteri,2004Nerium oleander L.).]]>136125137Golob, P., C. Moss, M. Dales, A. Fidgen, J. Evans and I. Gudrups,1999Pages: 239Pages: 239Stebbins, M.K.,1999Pages: 144Pages: 144Oleander, N.,1997Nerium oleander L.]]>1997Huang, K.C.,19982nd Edn.,Pages: 544Pages: 544Nellis, D.W.,1997Pages: 315Pages: 315Hardin, J.W. and J.M. Arena,19742nd Edn.,Pages: 194Pages: 194Osterloh, J., S. Herold and S. Pond,198224715961597Dart, R.C.,2004pp: 183-184pp: 183-184IUCN.,2005pp: 169-170pp: 169-170El-Seedi, H.R., R. Burman, A. Mansour, Z. Turki, L. Boulos, J. Gullbo and U. Goransson,2013Urginea maritima.]]>145746757Skidmore-Roth, L.,20094th Edn.,Pages: 768Pages: 768Nellis, D.W.,1994Pages: 160Pages: 160Dunn, D.E., D.N. He, P. Yang, M. Johansen, R.A. Newman and D.C. Lo,2011In vitro and in vivo neuroprotective activity of the cardiac glycoside oleandrin from Nerium oleander in brain slice-based stroke models.]]>119805814Raghavendra, P.B., Y. Sreenivasan and S.K. Manna,20074422922302Yang, P., C. Cartwright, E. Efuet, S.R. Hamilton and I.I. Wistuba et al.,2014+, K+-ATPase α subunits affect the anti-proliferative activity of oleandrin.]]>53253263Junior, P., D. Krueger and C. Winkler,198512519451949Begum, S., B.S. Siddiqui, R. Sultana, A. Zia and A. Suria,1999Nerium oleander.]]>50435438Yamauchi, T., F. Abe, Y. Tachibana, C.K. Atal, B.M. Sharma and Z. Imre,19832222112214Praveen, U.S., M.D. Gowtham, C.V. Yogaraje-Gowda, V.G. Nayak and B.M. Mohan,2012Nerium oleander by high-performance thin-layer chromatography in autopsy samples.]]>2135142Arao, T., C. Fuke, H. Takaesu, M. Nakamoto, Y. Morinaga and T. Miyazaki,200226222227Langford, S.D. and P.J. Boor,1996109113Hammiche, V., R. Merad and M. Azzouz,2013pp: 157-166pp: 157-166Yamauchi, T.,19741974Pellati, F., R. Bruni, M.G. Bellardi, A. Bertaccini and S. Benvenuti,2009Digitalis lanata.]]>121632603269Caporal-Gautier, J., J.M. Nivet, P. Algranti, M. Guilloteau and M. Hist et al.,19922205226, (In French)Larabi, I.L., M. Azzouz, R. Abtroun, M. Reggabi and B. Alamir,2012Atractylis gummifera L. collected from six different areas of Algeria].]]>248186, (In French)Karawya, M.S., S.I. Balbaa and S.E. Khayyal,1973Nerium oleander.]]>237073Roca-Perez, L., P. Perez-Bermudez and R. Boluda,2002Digitalis obscura wild populations.]]>2520152026Rufo, L., N. Rodriguez and V. de la Fuente,201195129139Burrows, G.E. and R.J. Tyrl,20122nd Edn.,pp: 81-127pp: 81-127