Research Journal of Phytochemistry1819-34712151-6081Science International10.3923/rjphyto.2017.129.141Mentha longifolia (L.) Huds]]>SinghPrakash KumarRavendra PrakashOm KumarMahesh PantAnil Kumar IsidorovValary A. SzczepaniakLech 32017113Background and Objective:Mentha longifolia is an extremely flavorful and aromatic herb of the mint family native to many parts of Europe, Asia and Northern and Southern Africa also known as horse mint. Extracts of M. longifolia have been used for centuries as a food flavoring and a medicine. The present study was aimed to reinvestigate the essential oil of Mentha longifolia (L.) Huds. for its chemical composition and concomitantly anti-inflammatory, analgesic, antipyretic, antibacterial and fungicidal activity. Materials and Methods: The essential oil was extracted using hydrodistillation method and analyzed by GC-MS. Anti-inflammatory, anti-nociceptive and antipyretic activities of essential oil were experimentally determined using mice model. Antibacterial activity and antifungal activity were determined by using disc diffusion and agar dilution method, respectively. Results were analyzed using one-way ANOVA followed by Dunnetts multiple comparison test. Results: The major constituents identified in the essential oil of Chamoli collection were piperitenone oxide (60.44%), 1,8-Cineole (6.16%), piperitenone (3.46%), dl-limonene (2.72%) and piperitone (1.69%). The Joshimath collection showed the presence of piperitenone oxide (36.89%), cis-piperitone oxide (17.89%), camphor (3.11%), isophorone (2.52%), thymol (2.41%) and coumarin (1.41%) and the Badrinath collection contains cis-piperitone oxide (42.74%), piperitenone oxide (33.48%), 4-Hydroxypiperitone (3.26%), thymol (2.52%), 2-Isobutylcyclopent-3-en-1-one (1.57%) and piperitenone (1.02%). The other constituents were present in minor quantities (less than 1.0%) in all three collections. The essential oil of Mentha longifolia show insignificant anti-inflammatory and analgesic activity but shows significant antipyretic, antibacterial and antifungal activity. Conclusion: The study indicated the wide chemical diversity in the essential oil of Mentha longifolia (L.) Huds collected from different geographical regions. The variation in chemical constituents may also effect the biological activities of the samples collected from different locations.]]>Chopra, R.N., I.C. Chopra, K.L. Handa and D.L. Kapur,2006pp: 196pp: 196Uniyal, B.P., J.R. Sharma, U. Choudhary and D.K. Singh,2007Pages: 404Pages: 404Amabeoku, G.J., S.J. Erasmus, J.A. Ojewole and J.T. Mukinda,2009Mentha longifolia Huds. (Lamiaceae) leaf aqueous extract in rats and mice.]]>31645649Conforti, F., S. Sosa, M. Marrelli, F. Menichini and G.A. Statti et al.,2008In vivo anti-inflammatory and in vitro antioxidant activities of Mediterranean dietary plants.]]>116144151Mkaddem, M., J. Bouajila, M. Ennajar, A. Lebrihi, F. Mathieu and M. Romdhane,2009Mentha longifolia L. and viridis) essential oils.]]>74M358M363Gulluce, M., F. Sahin, M. Sokmen, H. Ozer and D. Daferara et al.,2007Mentha longifolia L. ssp. longifolia.]]>10414491456Nimica-Dukic, N., M. Popovic, V. Jakovlijevic, A. Szabo and O. Gasic,1999Mentha longifolia phenolic extracts. II. Hepatoprotective activity.]]>37221224Mimica-Dukic, N., V. Jakovljevic, P. Mira, O. Gasic and A. Szabo,1996Mentha longifolia phenolic extracts.]]>34359364Shah, A.J., N.N. Bhulani, S.H. Khan, N. Rehman and A.H. Gilani,2010Mentha longifolia L. explains its medicinal use in diarrhoea and gut spasm.]]>2413921397Baris, O., M. Karadayi, D. Yanmis, Z. Guvenalp, T. Bal and M. Gulluce,2011Mentha longifolia (L.) Hudson subsp. longifolia and determination of their genotoxic potentials by using the E. Coli WP2 test system.]]>76T212T217Rasooli, I. and M.B. Rezaei,2002Zataria multiflora Boiss and Mentha longifolia (L.) Huds.]]>14141146Mimica-Dukic, N., B. Bozin, M. Sokovic, B. Mihajlovic and M. Matavulj,2003Mentha species essential oils.]]>69413419Hajlaoui, H., M. Snoussi, H.B. Jannet, Z. Mighri and A. Bakhrouf,2008Mentha longifolia L. ssp. longifolia essential oil from two Tunisian localities (Gabes and Sidi Bouzid).]]>2008Hajlaoui, H., N. Trabelsi, E. Noumi, M. Snoussi, H. Fallah, R. Ksouri and A. Bakhrouf,2009Mentha longifolia and Mentha pulegium) used in the Tunisian folkloric medicine.]]>2522272238Singh, G., P. Marimuthu, H.S. Murali and A.S. Bawa,200525130145Hafedh, H., B.A. Fethi, S. Mejdi, N. Emira and B. Amina,2010Mentha longifolia L. ssp. longifolia essential oil on the morphology of four pathogenic bacteria visualized by atomic force microscopy.]]>411221127Ali, M.S., W. Ahmed, M. Saleem and M.A. Ali,2006Mentha longifolia (Lamiaceae).]]>20715723Ali, M.S., M. Saleem, W. Ahmad, M. Parvez and R. Yamdagni,2002Mentha longifolia (Lamiaceae).]]>59889895Kouroussou, R., T. Lanara and S. Kokkini,1998Mentha longifolia subsp. petiolata and M. x villoso-nerveta grown on the Island of Crete (S Greece).]]>10375379Maffei, M.,1988Mentha longifolia (L.) Hudson particularly rich in piperitenone oxide.]]>32326Venskutonis, P.R.,1996Mentha longifolia L. from Lithuania rich in piperitenone oxide.]]>89195Sharopov, F.S., V.A. Sulaimonova and W.N. Setzer,2012Mentha longifolia from wild populations growing in Tajikistan.]]>17684Aksit, H., I. Demirtas, I. Telci and G. Tarimcilar,2013Mentha longifolia (L.) Hudson subsp. typhoides (Briq.) Harley var. typhoides from Turkey.]]>25430437Niksic, H., E.K. Besovic, E. Makarevic and K. Duric,2012Mentha longifolia (L.) Huds. essential oil.]]>2192200Fleisher, Z. and A. Fleisher,1998Mentha longifolia growing in Israel. Aromatic plants of the Holy Land and the Sinai. Part XIII.]]>10647648Koliopoulos, G., D. Pitarokili, E. Kioulos, A. Michaelakis and O. Tzakou,2010Mentha, Salvia and Melissa essential oils against the West Nile virus mosquito Culex pipiens.]]>107327335Melkani, A.B., G.C. Shah and R. Parihar,1990Mentha longifolia subsp. himalaiensis: A new species as a source of aroma chemicals.]]>1990pp: 177180Singh, H.P., D.R. Batish, S. Mittal, K.S. Dogra, S. Yadav and R.K. Kohli,2008Mentha longifolia from india.]]>44528529Kharakwal, H., G.C. Shah, C.S. Mathela and R. Laurent,1994Mentha longifolia subsp. himalaiensis.]]>382932Verma, R.S., V. Pandey, A. Chauhan and R. Tiwari,2015Mentha longifolia (L.) L. collected from Garhwal region of Western-Himalaya.]]>18957966Nori-Shargh, D., H. Norouzi-Arasi, S. Mohammadi, M. Mirza and K. Jaimand,2000Mentha longifolia (L.) Huds. from Iran.]]>12111112Abu-Al-Futuh, I.M., O.H. Abdelmageed, R.M. Jamil and P. Avato,2000Mentha longifolia (L.) Huds. growing wild in Jordan.]]>12530532Hussain, A.I.,20092009Baser, K.H.C., K.R. Nuriddinov, A.M. Nigmatullaev and K.N. Aripov,1997Mentha asiatica Boriss. from Uzbekistan.]]>9453454Ghoulami, S.A., I.L. Idrissi and S. Fkih-Tetouani,2001Mentha longifolia of Morocco.]]>72596598Kokkini, S., R. Karousou and T. Lanaras,199523425430Mastelic, J. and I. Jerkovic,2002Mentha longifolia growing in Croatia.]]>38561564Mathela, C.S., R.C. Padalia, C.S. Chanotiya and A. Tiwari,2005Mentha longifolia (Linn.): Chemical variation and commercial potential.]]>8130133Monfared, A., M.R. Nabid and A. Rustaiyan,2002Mentha longifolia (L.) Huds. from Iran.]]>145152Younis, Y.M.H. and S.M. Basher,2004Mentha longifolia (L.) Huds. ssp. schimperi Briq. and Mentha spicata L. grown in Sudan.]]>16539541Zeinali, H., A. Arzani, K. Razmjoo and M.B. Rezaee,2005Mentha ssp.).]]>17156159Dzamic, A.M., M.D. Sokovic, M.S. Ristic, M. Novakovic, S. Grujic-Jovanovic, V. Tesevic and P.D. Marin,2010Mentha longifolia (L.) Hudson (Lamiaceae) essential oil.]]>345761Viljoen, A.M., S. Petkar, S.F. Van Vuuren, A.C. Figueiredo, L.G. Pedro and J.G. Barroso,2006Mentha longifolia subsp. polyadena (Lamiaceae) in Southern Africa.]]>186065Oyedeji, A.O. and A.J. Afolayan,2006Mentha longifolia (L.) L. subsp. capensis (Thunb.) Briq.]]>185760Fleisher, A. and Z. Fleisher,1991Mentha longifolia growing in Sinai and Israel: Aromatic plants of the holy land and the Sinai. Part IV.]]>35758Golparvar, A.R., A. Hadipanah, M.M. Gheisari, S. Salehi, R. Khaliliazar and O. Ghasemi,2017Mentha longifolia (L.) Huds.]]>7235241Maisonneune, S.A.,1975Vol. 3.Adams, R.P.,20074th Edn.,Pages: 804Pages: 804Henriques, M.G., P.M. Silva, M.A. Martins, C.A. Flores, F.Q. Cunha, J. Assreuy-Filho and R.B. Corderio,198720243249Selye, H.,1949211291135Collier, H.O.J., L.C. Dinneen, C.A. Johnson and C. Schneider,196832295310Langerman, L., M.I. Zakowski, B. Piskoun and G.J. Grant,1995342327Rao, R.R., R.M. Babu, M.R.V. Rao and M.G.V. Babu,1997Gynandropsis gynandra Linn.]]>34690693Karaman, I., F. Sahin, M. Gulluce, H. Ogutcu, M. Sengul and A. Adiguzel,2003Juniperus oxycedrus L.]]>85231235Deba, F., T.D. Xuan, M. Yasuda and S. Tawatu,2008Bidens pilosa Linn. var. Radiata.]]>19346352Methella, C.S., G.C. Shah, A.B. Melkani and A.K. Pant,1989Mentha longifolia himalaiensis.]]>60349350Rezaei, M.B., K. Jaymand and Z. Jamzad,2000Mentha longifolia (L.) Hudson var. chlorodictya Rech. f. from three different localities.]]>136063Sharipova, F.S., L.A. Elchibekova, E.S. Nedeko and L.E. Gusak,1983Mentha arvensis L., Mentha longifolia (L.) Hudson, Mentha crispa L. and Mentha interrupta essential oils.]]>46771Hiruma-Lima, C.A., J.S. Gracioso, E.J.B. Bighetti, G.L. Robeneou and A.R.M.S. Brito,2000Boerhaavia diffusa L. (Nyctaginaceae) markedly reduces pain in mice.]]>71267274Helander, I.M., H.L. Alakomi, K. Latva-Kala, T. Mattila-Sandholm and I. Pol et al.,19984635903595Romagnoli, C., R. Bruni, E. Andreotti, M.K. Rai, C.B. Vicentini and D. Mares,2005Tagetes patula L.]]>2255765Guo, N., J. Liu, X. Wu, X. Bi and R. Meng et al.,2009Candida albicans.]]>5810741079Oumzil, H., S. Ghoulami, M. Rhajaoui, A. Ilidrissi, S. Fkih-Tetouani, M. Faid and A. Benjouad, 200216727731