Plant Pathology Journal1812-53871812-5425Asian Network for Scientific Information10.3923/ppj.2008.120.130Arabidopsis: A Possible Mechanism for Non-Seed Transmission]]>T. LarteyRobert GhoshroyKajal GhoshroySoumitra 2200872In this research we used Turnip vein clearing virus (TVCV)
to study viral non-transmission in A. thaliana as a step toward
developing a management strategy against seed transmissible viruses. Confocal
laser scanning, light, transmission and scanning electron microscopy and
immunolabeling techniques were used to study the pattern of the viral
movement in reproductive parts of the systemically infected plant. The
virus was located in all parts of the flower except ovules and pollen
grains. The viral movement was thus confined to the junction of ovule
and ovule stalk in the siliques and outside the pollen grains in anther
tissue. Subcellular localization of (1 > 3)- β -glucan (callose) indicated
selective deposition of this substance in plasmodesmata at the junction
of ovule and ovule stalk as well as exine layer of pollen grains. The
callose deposition was not observed in uninoculated and mock inoculated
control plants. We propose that the observed (1 > 3)- β -glucan may
be involved in blockage of viral movement into ovules and pollen grains
and result possibly in non-seed transmission of TVCV in A. thaliana.]]>Allison, A.V. and T.A. Shalla,197464784793Athow, K.L. and J.B. Bancroft,195949697701Beffa, R.S., R.M. Hofer, M. Thomas and F. Meins,1996810011011Botha, C.E.J. and R.H.M. Cross,200031713721Bucher, G. L., C. Tarina, M. Heinlein, F. Di Serio, F. Meins Jr. and V. Iglesius,200128361369Caroll, T.,19971997pp: 55-57pp: 55-57Collinge, D.B. and A.J. Slusarenko,19879389410Delmer, D.P., M. Volokita, M. Solomon, U. Fritz, W. Delphendahl and W. Herth,1993in vitro and colocalizes with site of high callose deposition in vivo.]]>1763342Edwards, M.C.,1995Barley stripe mosaic virus.]]>8906915Epstein, M.A. and S.J. Holt,196319325336Ghoshroy, S., R. Lartey, J. Sheng and V. Citovsky,1997482750Hartman, G.L., J.B. Sinclair and J.C. Rupe,19921st Edn.,Iglesias, V. and F. Meins, Jr.,200021157166Kendrick, J.B. and M.W. Gardner,1924279198Kuhn, C.W. and J.W. Demski,19841984pp: 45-46pp: 45-46Lartey, R., S. Ghoshroy, J. Ho and V. Citovsky,1997Arabidopsis.]]>12537545Lartey, R.T., S.D. Hartson, R.E. Pennington, J.L. Sherwood and U. Melcher,1993772124Lucas, W.J.,199950979987Mandahar, C.L.,19811981241-292241-292Matthews, R.E.F.,19913rd Edn.,Maule, A.J. and D. Wang,19964153158Meikle, P.J., I. Bonig, N.J. Hoogenraad, A.E. Clarke and B.A. Stone,199118518Mlcher, U.,20034133140Mink, G.I.,199331375402Olesen, P. and A.W. Robards,19901990145-170145-170Reynolds, E.S.,196317208212Sinclair, J.B.,19821st Edn.,Smith, K.M.,195116710611061Spurr, A.R.,1969263143Ueki, S. and V. Citovsky,20024478486Walker, J.C.,19691st Edn.Wang, D. and A.J. Maule,19946777787