Plant Pathology Journal1812-53871812-5425Asian Network for Scientific Information10.3923/ppj.2007.306.311ElwakilMohamed A.El-SherifEbtisam M.El-MetwallyMohamed A.4200764A method suitable for detecting the slow growing seed-borne fungi of peanut was developed. Twenty-five seed samples collected from commercial markets in Egypt were used in this investigation. With the Standard Moistened Blotter Method (SBM) and Deep-Freezing Blotter method (DFB) recommended by the International Seed Testing Association, saprophytes developed quickly and often impaired the detection of parasitic fungi and inhibited the germination of some important seed-borne fungi. Moistening the blotter disks used for seed germination with an alkaline solution at pH 12.5 using NaOH (0.8%) or KOH (0.4%) enhanced the growth and recovery of the slow growing seed-borne pathogens Cephalosporium sp. and Verticillium sp. The treatments also were effective in suppressing the growth of saprophytes which impair the detection of pathogenic fungi on seed. We recommend using the alkaline blotter method for seed health testing when searching for slow growing seed-borne fungi.]]>Abo-Ellil, A.H.A.,1999Verticillium lateritium.]]>2301304Baird, R.E., T.B. Brenneman, B.G. Mullinix, D.K. Bell, A.K. Culbreath and J.D. Moore,199377736741Baird, R.E., T.B. Brenneman, B.G. Mullinix, D.K. Bell, A.K. Culbreath and J.D. Moore,199377405408Booth, C.,1985Fusarium.]]>2nd Edn.,Pages: 237Pages: 237Burgess, L.W., C.M. Liddell and B.A. Summerell,1988Fusarium Research. Incorporating a Key and Descriptions of Common Species Found in Australia.]]>2nd Edn.,pp: 156pp: 156Chidambaram, P., S.B. Mathur and P. Neergaard,1973Drechslera species.]]>Pages: 207Pages: 207Dharmaputra, O.S. and I. Retnowati,199691525Duncan, D.B.,195511142Ellis, M.B.,19711st Edn.,Pages: 608Pages: 608Mohamed, E. and K.M. Ghoneem, 200211113Hollowell, J.E., B.B. Shew, M.K. Beute and Z.G. Abad,19988213451349Horikoshi, K. and T. Akiba,1982pp: 201pp: 201ISTA.,1996241335Mathur, S.K., S.B. Mathur and P. Neergaard,1975Fusarium moniliforme in seed.]]>3683690Melouk, H.A., D.F. Wadsworth and J.L. Sherwood,1983Verticillium wilt on root and top weight of peanut cultivar Tamnut 74.]]>6713491350Moubasher, A.H., I.A. El-Kady and S.M. Farghally,1977189141147Neergaard, P.,1979Vol. 1-2,pp: 1191pp: 1191Porter, D.M., D.H. Smith and R. Rodriguez-Kabana,1990Raper, K.B. and D.I. Fennell,1965Aspergillus.]]>Pages: 686Pages: 686Richardson, M.J.,19904th Edn.,Shim, H., D. Lee, G. Lee, H. Yu-Seung and H. Shim et al.,199638507515Singh, K., J.C. Frisvad, U. Thrance and S.B. Mathur,1991Aspergilli, Fusaria, Penicillia and their Mycotoxins.]]>Abo-Ellil, A.H.A. and N.S.I. Geweely,1999Penicillium Albicans (Alkalosensitive) and Verticillium lateritium (Facultative Alkalophile).]]>2290295