Plant Pathology Journal1812-53871812-5425Asian Network for Scientific Information10.3923/ppj.2007.227.234Tospovirus Causing Leaf and Stem Necrosis on Potato]]>GolnaraghiAli RezaPourrahimReza FarzadfarShirin AhoonmaneshAli 3200763A Tospovirus was isolated by mechanical sap transmission from potato plants showing leaf and stem necrosis symptoms and identified as Tomato yellow fruit ring virus (TYFRV) based on biological, serological and molecular studies. In mechanical transmission trials, a wide range of indicator plants, including members of the Amaranthaceae, Chenopodiaceae, Cucurbitaceae, Fabaceae and Solanaceae, closely similar to that of the not-infecting Nicotiana isolates of the same virus, was infected. The isolates under study strongly reacted with TYFRV antibodies, but not with the specific antibodies of the other tospoviruses tested (Groundnut ringspot virus, Impatiens necrotic spot virus, Iris yellow spot virus, Tomato chlorotic spot virus, Tomato spotted wilt virus and Watermelon silver mottle virus). In reverse transcription-polymerase chain reaction (RT-PCR), using specific primers for TYFRV-N gene, a DNA product with the expected size of approximately 1,200 bp was amplified from infected plants. The sequence analysis of this fragment showed a high identity with the corresponding region of TYFRV and confirmed the presence of this virus in Iranian potato crops. In this study, the transmission of TYFRV through potato tubers, even if at very low extent, was evidenced for the first time.]]>Abad, J.A., J.W. Moyer, G.G. Kennedy, G.A. Holmes and M.A. Cubeta,2005Tomato spotted wilt virus on potato in eastern North Carolina.]]>82255261Al-Shahwan, I.M., O.A. Abdalla and M.A. Al-Saleh,1997469194Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman,19972533893402Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl,1993Burland, T.G.,20001327191Clark, M.F. and A.N. Adams,197734475483Cortez, I., J. Saaijer, K.S. Wongjkaew, A.M. Pereira, R. Goldbach, D. Peters and R. Kormelink,2001146265278FAO.,20042004Fauquet, C.M., M.A. Mayo, J. Maniloff, U. Desselberger and L.A. Ball,2005Pages: 1162Pages: 1162German, T.L., D.E. Ulman and J.W. Moyer,199230315348Ghotbi, T., N. Shahraeen and S. Winter,200589425429Goldbach, R. and D. Peters,19945113120Granval de Milan, N., R. Piccolo and O. Gracia,1998Solanum tuberosum L.) tuber transmission of tospoviruses (TSWV, GRSV and TCSV) in Mendoza, Argentina.]]>1998pp: 4647Golnaraghi, A.R., R. Pourrahim, S. Farzadfar, K. Ohshima, N. Shahraeen and A. Ahoonmanesh,200761421Golnaraghi, A.R., R. Pourrahim, N. Shahraeen and S. Farzadfar,2002Groundnut bud necrosis virus in Iran.]]>86561561Golnaraghi, A.R., N. Shahraeen, R. Pourrahim and Sh. Farzadfar,2002Tomato spotted wilt virus isolates from soybean in Iran.]]>2002Hooker, W.J.,1990Jefferies, C.,1998Jozsef, H., R. Gaborjanyi, G. Kazinczi and A.P. Takacs,200150545547Khurana, S.M.P., S.K. Pandey, R.B. Singh and U.M. Bhale,1997132328Lathman, L.J. and R.A.C. Jones,199748359369Loenbenstein, G., P.H. Berger, A.A. Brunt and R.H. Lawson,2001Louro, D.,1996Tomato spotted wilt virus and impatiens necrotic spot virus in Portugal.]]>43199105Moini, A.A. and K. Izadpanah,2001Impatiens necrotic spot virus infection of tobacco in Iran.]]>379393Mound, L.,200550247269Moyer, J.W.,1999Bunyaviridae).]]>1999pp: 1803-1807pp: 1803-1807Moyer, J.W., T. German, J.L. Sherwood and D. Ulman,1999Tomato spotted wilt virus and related tospoviruses.]]>1999Mumford, R.A., I. Barker and K.R. Wood,1996128159183Nagata, T., A.C. de Avila, C.J. Barbosa, P.C.T. Melo, F.C. Juliatti and E.W. Kitajima,1995209095Parella, G., P. Gognalons, K. Gebre-Selassie, C. Volvas and G. Marchoux,2003Tomato spotted wilt virus.]]>85227264Perry, K.L., L. Miller and L. Williams,200589340340Prins, M. and R. Kormelink,19981998Thompson, J.D., D.G. Higgins and T.J. Gibson,19942246734680Whitfield, A.E., D.E. Ulman and T.L. German,200543459489Wilson, R.C.,2001Tomato spotted wilt virus in potatoes.]]>50402410Winter, S., M. Koerbler, N. Shahraeen, L. Katul and D.E. Lesemann,2002Tospovirus species infecting tomato in Iran.]]>2002pp: 120Winter, S., N. Shahraeen, M. Koerbler and D.E. Lesemann,2006Tospovirus species infecting tomato in Iran.]]>55287287