Plant Pathology Journal1812-53871812-5425Asian Network for Scientific Information10.3923/ppj.2006.401.404Datura stramonium L. By an Unusual Strain of Pepper veinal mottle virus Genus Potyvirus in Nigeria]]>TaiwoM.A. HughesJ.d`A. AkomahA.R. 3200653A disease causing mosaic and leaf distortion was observed in 80% of Datura stramonium L. plants in a garden in Ibadan, Nigeria. The causal agent had flexuous rod-shaped particles of about 750-800 nm length. The virus was readily mechanically transmissible but was not seed borne in the natural host. The virus was not transmissible to any other host genera and was restricted to D. stramonium and D. metel. In serological tests using protein A-sandwich enzyme-linked immunosorbent assay (PAS-ELISA) and antisera to ten members of the family Potyviridae, the virus from D. stramonium reacted only with the antiserum to Pepper veinal mottle virus(PVMV) genus Potyvirus. Optical density readings of 3.564 and 0.466 at 405 nm were obtained in reactions between PVMV antiserum and virus infected and healthy Datura sp.samples respectively. No serological relationship was detected between the virus from D. stramonium and Blackeye cowpea mosaic virus (BICMV) genus Potyvirus, Cowpea aphid-borne mosaic virus (CABMV) genus Potyvirus and Yam mosaic virus (YMV) genus Potyvirus in double antibody sandwich ELISA (DAS-ELISA) and immunosorbent electron microscopy (ISEM) tests. Although some of the data on symptomatology, host range and serological relatedness could implicate Datura shoestring virus (DSV) genus Potyvirus, the virus may actually be an unusual strain of PVMV.]]>Atiri, G.I.,1984104261265Brunt, A., K. Crabtree and A. Gibbs,1990Fauquet, M.C. and M.A. Mayo,1999612491273Giri, L. and H.O. Agrawal,1971Datura shoestring virus, a new virus isolated from Solanum jasminoides.]]>708083Hobbs, H.A., D.V.R. Reddy, R. Rajeswari and A.S. Reddy,198771747749Huguenot, C., M.T. Furneaux, G. Thottappilly, H.W. Rossel and R.I. Hamilton,199374335340Igwegbe, E.C.K. and H.E. Waterworth,1982Solanum melongena L.) in Nigeria.]]>103912Kahn, R.P. and R. Bartles,1968Columbian datura virus, a new virus in the potato virus Y group.]]>58587592Ladipo, J.L. and I.M. Roberts,1979Pepper veinal mottle virus in tobacco in Nigeria.]]>63161165Ladipo, J.L.,1987Pepper veinal mottle virus isolated from tomato in Nigeria.]]>112432Lana, A.O., R.M. Gilmer, G.F. Wilson and S.A. Shoyinka,1975Pepper veinal mottle virus isolated from pepper (Capsicum annuum and C. fructescens) in Nigeria.]]>17681Prasanna, G.B., A. Rajeswari, T. Satyanarayana, P. Sreenivasulu and M.V. Nayudu,1996Datura metel in India.]]>12129135Richter, J., E. Proll, F. Rabenstein and A. Stanarius.1994Potyviridae with polyclonal antisera.]]>1421118Taiwo, M.A., A. Hughes and A.R. Akomah,2004D. stramonium L. by an unusual strain of PVMV in Nigeria.]]>9466Loebenstein, G. and G. Thottappilly,2003pp: 800pp: 800Ladipo, J.L. and I.M. Roberts,197787133138Ivens, A.W., K. Moody and J.K. Egunjobi,1978pp: 225pp: 225Brunt, A.A. and R.H. Kenten,19721972Thonner, F.,1962pp: 647pp: 647