Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2015.37.41In vivo Induction of Tetraploid in Tangerine Citrus Plants (Citrus reticulata
Blanco) with the Use of Colchicine]]>SursonSuntaree SitthaphanitSuphasit WongmaNattapong 12015181This in vivo experiment was carried out at Sakhon Nakhon Rajabhat University,
Sakhon Nakhon Province, Thailand during March-October 2013. The study aims to
search for some possibilities in inducing a large number of tetraploid sets
of chromosomes in tangerine citrus seedlings with the use of colchicine chemical.
A Randomized Complete Block Design (RCBD) with four replications was used. Seeds
of tangerine citrus were treated with colchicine solutions. The experiment consisted
of seven treatments, i.e., T1 with 0.0% colchicine (control), T2 with 0.2% colchicine
solution and submerged for 12 h, T3 with 0.2% colchicine solution and submerged
for 24 h, T4 with 0.4% colchicine solution and submerged for 12 h, T5 with 0.4%
colchicine solution and submerged for 24 h, T6 with 0.8% colchicine solution
and submerged for 12 h, T7 with 0.8% colchicine solution and submerged for 24
h. The experiment was conducted for 91 days. The results showed that colchicine
compound severely and significantly affected germination of tangerine citrus
seeds. Colchicine of 0.2% in the solution with seeds submerged for 24 h gave
the highest percentages of tetraploid chromosomes in seedlings of tangerine
citrus (63.64%). Colchicine significantly affected seed germination, plant height,
stomata density and leaf index of the tangerine citrus seeds and seedlings.
Colchicine had no significant effect on poly-embryos, mono-embryos, leaf number,
leaf area, leaf weight, leaf length and stomata length of the tangerine citrus
seedlings.]]>Agrawal, R.L.,1998Pages: 394Pages: 394Blanke, M.M., M. Hofer and R.J. Pring,1994in-vitro.]]>73651654Chinachit, W. and S. Sreemaung,2008Eulophia andamanensis Reichb.f].]]>39275277, (In Thai)Daughtry, C.S.T.,199054560Dhamayanthi, K.P.M. and V. Gotmare,2010G. armourianum and G. aridum) species by colchicine treatment.]]>1 966972Van Duren, M., R. Morpurgon, J. Dolezel and R. Afza,1996Musa acuminata) by in vitro techniques.]]>882534Fassuliotis, G. and B.V. Nelson,1992117863866Gmitter, Jr. F.G. and X. Ling,1991in vitro and nonchimeric tetraploid plant recovery from undeveloped citrus ovules treated with colchicine.]]>116317321Grouh, M.S.H., H. Meftahizade, N. Lotfi, V. Rahimi and B. Baniasadi,2011Salvia hains using colchicine: Evaluation of morphological traits of recovered plants.]]>548924898Hill, J.B., H.W. Poop and A.R. Grove Jr.,19674th Edn.,Pages: 614Pages: 614Kerdsuwan, N. and S. Te-chato,2012Rhynchostylis gigantean var. rubrum Sagarik) in vitro.]]>814511460Majdi, M., G. Karimzadeh, M.A. Malboobi, R. Omidbaigi and G. Mirzaghaderi,2010Tanacetum parthenium Schulz-Bip.): Morphological, physiological, cytological and phytochemical changes.]]>451621Petersen, K.K., P. Hagberg and K. Kristiansen,2003Miscanthus sinensis.]]>73 137146Pirkoohi, M.H., M. Keyvanloo and M. Hasanpoor,20113-4102104Verheij, E.W.M. and R.E. Coronel,1992Wongpiyasatid, A., P. Hormchan and N. Rattanadilok,2003Gossypium arboretum) using colchicine treatment.]]>372732SAS.,1998Version 6,Suksri, A.,19991st Edn.,Pages: 212Pages: 212