Pakistan Journal of Biological Sciences
1028-8880
1812-5735
Asian Network for Scientific Information
10.3923/pjbs.2014.802.811
Wani
Mohd Yaqoob
Dhama
Kuldeep
Latheef
Shyma K.
Barathidassan
Rajamani
Tiwari
Ruchi
Chakraborty
Sandip
Chawak
Milind Madhukar
Singh
Shambhu Dayal
6
2014
17
6
Chicken Infectious Anaemia Virus (CIAV) is one of the potent
immunosuppressive and economically important agents affecting poultry industry
worldwide. Recent reports indicate the emergence of this virus in the poultry
flocks of the country. The present study aimed to investigate the pathogenic
potential of a recent isolate of CIAV obtained from poultry flock of Uttaranchal
State, India. Twenty first day-old age Specific Pathogen Free (SPF) chicks were
inoculated intramuscularly with 104.5 median tissue culture infective
dose (TCID50) of CIAV passaged in the Mareks
disease virus transformed chicken splenic T lymphocyte (MDCC-MSB1) cell line
while 15 chicks were kept as control. The CIAV isolate produced consistent clinical
signs, loss in body weight gain, anaemia, low haematocrit values, bone marrow
aplasia and generalized lymphoid atrophy. Mean Packed Cell Volume (PCV) value
of the infected chicks was significantly low (18.22±2.22) compared to
control group (34.12±4.72) at 14 day post infection (dpi). The establishment
of virus infection in chicks was confirmed both at molecular and antigenic levels
by Polymerase Chain Reaction (PCR) and Indirect Immunofluorescent Test (IIFT),
respectively. Characteristic apoptotic pattern was also detected in the affected
organs and the virus was re-isolated successfully in MDCC-MSB1 cell cultures.
The present results revealed that the virus circulating in poultry flocks of
Uttaranchal state is both pathogenic and immunosuppressive in nature. Extensive
epidemiological studies are suggested in the poultry flocks of the country along
with adaptation of appropriate diagnostic, prevention and control strategies
so as to prevent economic losses caused by this important poultry pathogen.]]>
[
Adair, B.M.,
2000
24
247
255
]
[
Basaraddi, M.S., K. Dhama, M.Y. Wani, P.M. Sawant and R. Tiwari et al.,
2013
7
2464
2474
]
[
Bhatt, P., S.K. Shukla, M. Mahendran, K. Dhama, M.M. Chawak and J.M. Kataria,
2011
58
458
460
]
[
Bhatt, P., S.K. Shukla, K. Dhama and A.K. Thathoo,
2011
12
90
93
]
[
Bhatt, P., S.K. Shukla, M.Y. Wani, R. Tiwari and K. Dhama,
2013
2013
]
[
Brown, K., G.F. Browning, P.C. Scott and B.S. Crabb,
2000
78
637
640
]
[
Campbell, T.W.,
1995
2nd Edn.,
]
[
Connor, T.J., F. McNeilly, G.A. Firth and M.S. McNulty,
1991
68
199
201
]
[
Dhama, K.,
2002
2002
]
[
Dhama, K., J.M. Kataria, B.B. Dash, N.S. Kumar and S. Tomar,
2002
23
1
15
]
[
Dhama, K., J.M. Kataria, N. SenthilKumar, B.B. Dash, S. Tomar and G.R. Ghalsasi,
2003
38
185
194
]
[
Dhama, K., J.M. Kataria, N. Senthilkumar and S. Tomar,
2004
25
75
79
]
[
Dhama, K., M. Mahendran, R. Somvanshi and M.M. Chawak,
2008
32
158
167
]
[
Dhama, K., M. Mahendran, P.K. Gupta and A. Rai,
2008
32
341
356
]
[
Dhama, K., P.M. Sawant, D. Kumar and R. Kumar,
2011
6
32
40
]
[
Dhama, K., K. Karthik, S. Chakraborty, R. Tiwari, S. Kapoor, A. Kumar and P. Thomas,
2014
17
151
166
]
[
Dhama, K., A.K. Verma, R. Tiwari, S. Chakraborty and K. Vora et al.,
2013
1
14
24
]
[
Dhama, K., S. Chakraborty, Mahima, M.Y. Wani and A.K. Verma et al.,
2013
16
101
111
]
[
Dhama, K., S. Chakraborty and R. Tiwari,
2013
3
170
178
]
[
Dhama, K., S. Chakraborty, M.Y. Wani, R. Tiwari and R. Barathidasan,
2013
3
195
208
]
[
Dhama, K., S. Chakraborty, S. Kapoor, R. Tiwari and A. Kumar et al.,
2013
1
5
13
]
[
Ducatez, M.F., H. Chen, Y. Guan and C.P. Muller,
2008
52
68
73
]
[
Ducatez, M.F., A.A. Owoade, J.O. Abiola and C.P. Muller,
2006
151
97
111
]
[
Dos Santos, H.F., M.B. Knak, F.L. de Castro, J. Slongo and G.A. Ritterbusch et al.,
2012
155
230
236
]
[
Goryo, M., S. Hayashi, K. Yoshizawa, T. Umemura, C. Itakura and S. Yamashiro,
1989
18
605
617
]
[
Goryo, M., T. Suwa, T. Umemura, C. Itakura and S. Yamashiro,
1989
18
329
343
]
[
Gowthaman, V., S.D. Singh, K. Dhama, R. Barathidasan and M.A. Kumar et al.,
2012
15
900
903
]
[
Hagood, L.T., T.F. Kelly, J.C. Wright and F.J. Hoerr,
2000
44
803
808
]
[
Hailemariam, Z., A.R. Omar, M. Hair-Bejo and T.C. Giap,
2008
5
128
128
]
[
Haridy, M., J. Sasaki, K. Okada and M. Goryo,
2012
93
1353
1360
]
[
He, C.Q., N.Z. Ding, W. Fan, Y.H. Wu, J.P. Li and Y. Li,
2007
366
1
7
]
[
Hoop, R.K.,
1992
21
493
501
]
[
Hu, L.B., B. Lucio and K.A. Schat,
1993
+ and CD8+ T lymphocyte subpopulations by CIA-1, a chicken infectious anemia virus.]]>
37
492
500
]
[
Jeurissen, S.H.M., F. Wagenaar, J.M. Pol, A.J. van der Eb and M.H.M. Noteborn,
1992
in vivo infection and of cell lines after in vitro infection.]]>
66
7383
7388
]
[
Kataria, J.M., K. Dhama, B.B. Dash, S.A. Sylvester and S. Rahul et al.,
2005
2005
]
[
Kataria, J.M., R.P. Suresh, K.C. Verma, R. Toroghi, N.S. Kumar, R.S. Kataria and R.L. Sah,
1999
20
91
95
]
[
Kim, H.R., Y.K. Kwon, Y.C. Bae, J.K. Oem and O.S. Lee,
2010
89
2426
2431
]
[
Latheef, S.K., K. Dhama, M.Y. Wani, H.A. Samad and R. Barathidasan et al.,
2013
Withania somnifera, Tinospora cordifolia, Azadirachta indica and E care SE herbal) on the pathogenesis of chicken infectious anaemia virus.]]>
5
2327
2331
]
[
Liu, Z.G., S.M. Zheng, Y.B. Xu, Z.Y. Zhou, X.R. Wang and L.P. Yang,
1997
30
74
82
]
[
Mahima, A. Rahal, R. Deb, S.K. Latheef and H.A. Samad et al.,
2012
15
754
774
]
[
McNulty, M.S.,
1991
20
187
203
]
[
McNulty, M.S., S.G. McIlroy, D.W. Bruce and D. Todd,
1991
35
263
268
]
[
Miller, M.M. and K.A. Schat,
2004
48
734
745
]
[
Mohamed, M.A.,
2010
9
591
598
]
[
Natesan, S., J.M. Kataria, K. Dhama, S. Rahul and N. Baradhwaj,
2006
Chicken anaemia virus isolates of Indian origin.]]>
118
78
86
]
[
Natesan, S., J.M. Kataria, K. Dhama, N. Bhardwaj and A. Sylvester,
2006
87
2933
2940
]
[
Nayabian, H. and K. Mardani,
2013
42
108
113
]
[
Oluwayelu, D.O., D. Todd, N.W. Ball, A.N.J. Scott and O.A. Oladele et al.,
2005
49
446
450
]
[
Oluwayelu, D.O.,
2010
9
2043
2049
]
[
Pope, C.R.,
1991
30
51
65
]
[
Pringle, C.R.,
1999
144
2065
2070
]
[
Schat, K.A.,
2003
2003
pp: 182
pp: 182
]
[
Smyth, J.A., D.A. Moffett, M.S. McNulty, D. Todd and D.P. Mackie,
1993
37
324
338
]
[
Snoeck, C.J., G.F. Komoyo, B.P. Mbee, E. Nakouné, A. Le Faou, M.P. Okwen and C.P. Muller,
2012
2012
]
[
Tiwari, R., S. Chakraborty, K. Dhama, M.Y. Wani, A. Kumar and S. Kapoor,
2013
2013
]
[
Todd, D.,
2000
29
373
394
]
[
Venugopalan, A.T., S. Elankumaran, G.D. Raj, B.M. Manohar and A. Thangavelu,
1994
71
411
412
]
[
Verma, K.C., A.S. Panisup, G.C. Mohanty and B.C. Reddy,
1981
16
385
392
]
[
Verma, S., R.C. Katoch, A. Mahajan, M. Sharma, V. Katoch, J.M. Kataria and K. Dhama,
2005
82
119
122
]
[
Yuasa, N.,
1992
21
315
319
]
[
Dhama, K., M.Y. Wani, R. Deb, K. Karthik and R. Tiwari et al.,
2013
2013
]