Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2014.402.407In vitro Antioxidant Properties of Fucoidan Fractions From Sargassum
tenerrimum]]>MarudhupandiT. KumarT.T. Ajith SenthilS. Lakshmana DeviK. Nanthini 32014173The aim of the present study is conducted to evaluate the
antioxidant potential and toxicity effect of polysaccharide-fucoidan from the
brown seaweed Sargassum tenerrimum. Fucoidan-a sulphated polysaccharide
contains fucose and sulphate as major compounds. Interestingly, various studies
reported that the presence of sulphate content of the sample plays a significant
role in pharmacological activities. In this study, fucoidan was fractionated
by Ion exchange chromatography method and it major chemical constituent sulphate
and fucose was determined by the biochemical methods. The toxicity effect of
fucoidan was analyzed by the brine shrimp toxicity assay. Three fucoidan fractions
(F1, F2 and F3) were obtained from Intact Fucoidan (IF) through anion-exchange
column chromatography. In vitro antioxidant capability was analyzed by
1, 1-diphenyl-2-picrylhydrazyl (DPPH), superoxide radical scavenging and total
antioxidant assays and intact fucoidan showed the maximum activity 83.66±0.35,
81.73±0.35% and 41.6±0.43 mg g-1, respectively. The
finding of the present study was confirmed that the antioxidant property of
fucoidan was depending upon the sulphate content of the fraction and these studies
proved that fucoidan have non toxicity effect. Hence, fucoidan have the scope
of being used as natural antioxidants in treating many human diseases.]]>Beress, A., O. Wassermann, S. Tahhan, T. Bruhn and L. Beress et al.,1993Fucus vesiculosus.]]>56478488Bilan, M.I., A.A. Grachev, A.S. Shashkov, N.E. Nifantiev and A.I. Usov,2006Fucus serratus L.]]>341238245Bilan, M.I., A.A. Grachev, N.E. Ustuzhanina, A.S. Shashkov, N.E. Nifantiev and A.I. Usov,2002Fucus evanescens C.Ag.]]>337719730Chandini, S.K., P. Ganesan and N. Bhaskar,2008In vitro antioxidant activities of three selected brown seaweeds of India.]]>107707713Chattopadhyay, N., T. Ghosh, S. Sinha, K. Chattopadhyay, P. Karmakar and B. Ray,2010Turbinaria conoides: Structural features and antioxidant capacity.]]>118823829Chizhov, A.O., A. Dell, H.R. Morris, S.M. Haslam and R.A. McDowell,1999Chorda filum.]]>320108119Costa, L.S., G.P. Fidelis, S.L. Cordeiro, R.M. Oliveira and D.A. Sabry et al.,2010642128Deciga-Campos, M., I. Rivero-Cruz, M. Arriaga-Alba, G. Castaneda-Corral, G.E. Angeles-Lopez, A. Navarrete and R. Mata,2007110334342DuBois, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers and F. Smith,195628350356Hu, T., D. Liu, Y. Chen, J. Wu and S. Wang,2010Undaria pinnitafida in vitro.]]>46193198Lee, H.S., S.H. Jin, H.S. Kim and B.H. Ryu,1995Gompi, Ecklonia stolonifera.]]>27716723Lee, J., N. Koo and D.B. Min,200432133Li, B., F. Lu, X. Wei and R. Zhao,20081316711695Lim, S. N., P.C.K. Cheung, V.E.C. Ooi and P.O. Ang,2002Sargassum siliquastrum.]]>5038623866McClure, M.O., J.P. Moore, D. Blanc, P. Scotting and G.M.W. Cook et al.,1992in vitro.]]>81926Middleton, Jr. E., C. Kandaswami and T.C. Theoharides,200052673751Nagumo, T., T. Nishino and S. Dumitriu,1997pp: 545-574pp: 545-574Nishikimi, M., N.A. Rao and K. Yagi,197246849854Nishino, T., T. Nagumo, H. Kiyohara and H. Yamada,1991Ecklonia kurome.]]>2117790Painter, T.J.,198355677694Parra, A.L., R.S. Yhebra, I.G. Sardinas and L.I. Buela,2001Artemia salina L. and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts.]]>8395400Prieto, P., M. Pineda and M. Aguilar,1999269337341Saito, K., M. Nishijima and T. Miyazaki,19903817451747Shimada, K., K. Fujikawa, K. Yahara and T. Nakamura,199240945948Smit, A.J.,200416245262Usov, A.I. and A.V. Kiryanov,1994Laminaria cichroriodes miyabe.]]>2013421348Wang, J., Q. Zhang, Z. Zhang, H. Song and P. Cheng,2010Laminaria japonica.]]>46612Yan, X., T. Nagata and X. Fan, 199852253262Yang, C., D. Chung and S.G. You,2008Undaria pinnatifida using light scattering technique.]]>111503507Yoshizawa, Y., A. Ametani, J. Tsunehiro, K. Nomura, M. Itoh, F. Fukui and S. Kaminogawa,1995Prophyra yezoensis): Structure-function relationships and improved solubility.]]>5919331937 Zhang, Q., P. Yu, Z. Li, H. Zhang, Z. Xu and P. Li,2003Porphyra haitanesis.]]>15305310Zhang, Y., X. Lu, Z. Fu, Z. Wang and J. Zhang,2011Diospyros kaki L.) fruit and antioxidant activities of the sulphated derivatives.]]>12710841090