Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2014.380.387Mentha pulegium During Growth]]>ShahmohamadiRezvan SaririReyhaneh RasaMahdi AghamaliMahmudreza 32014173Antioxidant activity and total phenolic contents of methanolic and hydroalcoholic-acetone extracts of Iranian Mentha pulegium in two stages of maturity were investigated. The aim was to investigate the most suitable solvent for extraction of antioxidants and to find the correlation existed between plant growth stage and its antioxidant capacity. In vitro antioxidant properties of the extracts were examined by 1, 1 diphenyl-2-picrylhydrazyl (DPPH) and Ferric Reducing/Antioxidant Power (FRAP) methods. Moreover, total phenolic contents were determined by the Folin-Ciocalteu method. On the other hand, the phenolic compounds were analyzed by chromatographic methods, TLC and SPE-reversed phase-HPLC. The results revealed that the antioxidant capacities and total phenol contents of the extracts in flowering season were higher than pre-flowering season. A positive relationship was found between the results obtained from three different assay methods used, i.e. FRAP, DPPH and phenol contents. The TLC chromatogram of the two extracts showed differences in the number of separated compounds of extracts. HPLC results indicated that the fraction collected with washing buffer (pH = 6) had highest antioxidant activity.]]>Ali, S.S., N. Kasoju, A. Luthra, A. Singh, H. Sharanabasava, A. Sahu and U. Bora,200841115Atmani, D., N. Chaher, M. Berboucha, K. Ayouni and H. Lounis et al.,2009112303309Tepe, B., D. Daferera, A. Sokmen, M. Sokmen and M. Polissiou,2005Salvia tomentosa Miller (Lamiaceae).]]>90333340Benzie, I.F.F. and J.J. Strain,19992991527Bolton, J.L., M.A. Trush, T.M. Penning, G. Dryhurst and T.J. Monks,200013135160Brand-Williams, W., M.E. Cuvelier and C. Berset,1995282530Carnat, A., D. Fraisse, A.P. Carnat, C. Felgines, D. Chaud and J.L. Lamaison,200585598602Chalchat, J.C., M.S. Gorunovlc, Z.A. Maksimovlc and S.D. Petrovlc,2000Mentha pulegium L from Yugoslavia.]]>12598600Capecka, E., A. Mareczek and M. Leja,2005Lamiaceae species.]]>93223226Diandian, S.,2008Origanum ssp and Mentha spp. and analytical methods on the quality control of bioactive phenolic componds.]]>2008Fletcher, S.R., T. Slimmon and S.L. Kott,2010Mentha spicata L.) and peppermint (Mentha piperita L.).]]>41016Gao, X., M. Ohlander, N. Jeppsson, L. Björk and V. Trajkovski,2000Hippophae rhamnoides L.) during maturation.]]>4814851490Ghasemnezhad, M., M. Sherafati and G.A. Payvast,2011Capsicum annum) fruits at two different harvest times.]]>34449Guidi, I., D. Galimberti, S. Lonati, C. Novembrino and F. Bamonti et al.,200627262269Hai, W., H. Cheng, M. Zhao, Y. Wang, L. Hong, H. Tang and X. Tian,2012Clematis argentilucida.]]>83759764Van Horssen, J., G. Schreibelt, J. Drexhage, T. Hazes, C.D. Dijkstra, P. Van der Valk and H.E. De Vries,20084517291737Ismail, A., Z.M. Marjan and C.W. Foong,200487581586Ito, N., S. Fukushima, A. Haqlwara, M. Shibata and T. Ogiso,198370343347Kamkar, A., A.J. Javan, F. Asadi and M. Kamalinejad,2010Mentha pulegium extracts and essential oil in sunflower oil.]]>4817961800Katalinic, V., M. Milos, T. Kulisic and M. Jukic,200694550557Kim, D.O. and C.Y. Lee,200444253273Kosar, M., H.J.D. Dorman and R. Hiltunen,2005Lamiaceae species.]]>91525533Kris-Etherton, P.M., K.D. Hecker, A. Bonanome, S.M. Coval and A.E. Binkoski et al.,20021137188Kumar, K.V. and D.D. Patra,2012Mentha piperita L. plant: Effect of fly ash amendments and organic wastes.]]>47237241Mahboubi, M. and G. Haghi,2008Mentha pulegium L. essential oil.]]>119325327Matthaus, B.,200745483527Matkowski, A., P. Tasarz and E. Szypuła,2008Lamiaceae, subfamily Lamioideae.]]>2321330Moure, A., D. Franco, J. Sineiro, H. Domnguez, M.J. Nunez and J.M. Lema,1999Gevuina avellanahulls.]]>1999Mozaffarian, V.,1996pp: 198pp: 198Nickavar, B., A. Alinaghi and M. Kamalinejad,2008Mentha species.]]>7203209Papageorgiou, V., C. Gardeli, A. Mallouchos, M. Papaioannou and M. Komaitis,2008Rosmarinus officinalis L. and Salvia fruticosa miller grown in Greece.]]>5672547264Rahmat, A., V. Kumar, L.M. Fong, S. Endrini, H. Abdullah Sani,200312308311Shahmohamadi, R., R. Sariri, M. Rasa, H. Ghafoori, M. Aghamali, S. Nasuti and M. Tahery,20113651659Shirazi, F.H., N. Ahmadi and M. Kamalinejad,2004Mentha pulegium L.]]>12106110Smith, M.A., C.A. Rottkamp, A. Nunomura, A.K. Raina and G. Perry,20001502139144Spigno, G., L. Tramelli and D.M. De Faveri,200781200208Sultana, B., F. Anwar and R. Przybylski,2007Azadirachta indica, Terminalia arjuna, Acacia nilotica and Eugenia jambolana Lam. trees.]]>10411061114Li, J.W., S.D. Ding and X.L. Ding,20054036073613Wang, J., X. Yuan, Z. Jin, Y. Tian and H. Song,2007104242250Wojdylo, A., J. Oszmianski and R. Czemerys,2007105940949Zargari, A.,19901st Edn.,