Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2014.1015.1022Bacillus KKU02 and KKU03
and the Effects of the Produced Spores on Growth of Giant Freshwater Prawn (Macrobrachium
rosenbergii de Man)]]>Wangka-OrmCharkrit DeeseenthumSirirat LeelavatcharamasVichai 82014178In order to extend the shelf life of 2 high potential Bacillus
probiotic isolates which were Bacillus KKU02 and Bacillus KKU03,
the spore forms of these 2 Bacillus isolates were studied for using as
probiotic instead. The low cost medium for spore production of these 2 Bacillus
isolates was examined in order to produce probiotic spores for feeding the shrimps.
It was found that cassava at 100 g L-1 and supplemented with 20.0
g L-1 dextrose, 0.1 g L-1 MgSO4 and 2.0 g L-1
(NH4)2SO4 showed the highest spore concentration
at about 1x108 CFU mL-1. The effects of feeding these
2 Bacillus spores on the growth of giant freshwater prawns were further
examined. The spores of Bacillus KKU02 and Bacillus KKU03 (~107
spore mL-1) in pure and mixed culture forms were mixed with
commercial prawn feed (200 mL kg-1) to give six feed treatments.
Body length and weight of the prawns in mixed spore culture tanks after rearing
for 90 days (13.5 cm and 59.8 g, respectively) were significantly higher (p
= 0.05) than other treatments. The treated prawns were further challenged with
Aeromonas hydrophila for 7 days. The percentages of survival after the
challenge in the prawns fed with the mixed spores (46.8%) were also found significantly
higher (p = 0.05) than others groups, except the mixed live cell treatment (60%).
These results indicated that the spores of Bacillus KKU02 and Bacillus
KKU03 had a high potential for using as commercial probiotics.]]>Amaha, M., Z.J. Ordal and A. Touba,1956Bacillus coagulans var. Thermoacidurans in complex media.]]>723441Armstrong, D.A., M.J. Stephenson and A.W. Knight,1976Macrobrachium rosenbergii.]]>93946Austin, B., L.F. Stuckey, P.A.W. Robertson, I. Effendi and D.R.W. Griffith, 1995Vibrio alginolyticus effective in reducing diseases caused by Aeromonas salmonicida, Vibrio anguillarum and Vibrio ordalii.]]>189396Bachere, E.,2003227427438Balcazar, J.L., I. de Blas, I. Ruiz-Zarzuela, D. Cunningham, D. Vendrell and J.L. Muzquiz,2006114173186Bly, J.E., S.M.A. Quiniou, L.A. Lawson and L.W. Clem,1997Pseudomonas fluorescens.]]>203540Boyd, C. and S. Zimmerman,20002000pp: 221-238pp: 221-238Cartman, S.T. and R.M. La Ragione,20042004pp. 155-161pp. 155-161Casula, G. and S.M. Cutting,2002Bacillus probiotics: Spore germination in the gastrointestinal tract.]]>6823442352Cote, R. and R.L. Gherna,1999E. Coli and Bacillus Spp.]]>1999pp: 1676-1683pp: 1676-1683Curran, H.R.,19581958pp: 1-9pp: 1-9Cutting, S.M.,201128214220D'Arienzo, R., F. Maurano, G. Mazzarella, D. Luongo, R. Stefanile, E. Ricca and M. Rossi,2006Bacillus subtilis spores reduce susceptibility to Citrobacter rodentium-mediated enteropathy in a mouse model.]]>157891897Dechmahitkul, W., C. Youkong, K. Poomputra, S. Akeprathumchai and P. Mekvijitsaeng,2007Bacillus subtilis spores for animal probiotics.]]>30251259Deeseenthum, S., V. Leelavatcharamas and J.D. Brooks,2007Bacillus sp. as probiotic bacteria on growth of giant freshwater prawn (Macrobrachium rosenbergii de Man).]]>1014811485Gatesoupe, F.J.,1994Scophthalmus maximus, against pathogenic Vibrio sp.]]>7277282Gram, L., J. Melchiorsen, B. Spanggaard, I. Huber and T.F. Nielsen,1999Vibrio anguillarum by Pseudomonas fluorescens AH2, a possible probiotic treatment of fish.]]>65969973Hoa, T.T., L.H. Duc, R. Isticato, L. Baccigalupi, E. Ricca, P.H. Van and S.M. Cutting,2001Bacillus subtilis spores in a murine model.]]>6738193823Hong, H.A., L.H. Duc and S.M. Cutting,200529813835Hong, H.A., J.M. Huang, R. Khaneja, L.V. Hiep, M.C. Urdaci and S.M. Cutting,2008Bacillus subtilis and Bacillus indicus as food probiotics.]]>105510520Itami, T., M. Asano, K. Tokushige, K. Kubano and A. Nakawaga et al.,1998Penaeus japonicus, after oral administration of peptidoglycan derived from Bifidobacterium thermophilum.]]>164277288Kang, B.C., S.Y. Lee and H.N. Chang,1992Bacillus thuringiensis by fed-batch culture.]]>14721726Kolodziej, B.J. and R.A. Slepecky,1964Bacillus megaterium.]]>88821830La Ragione, R.M., G. Casula, S.M. Cutting and M.J. Woodward,2001Bacillus subtilis spores competitively exclude Escherichia coli O78:K80 in poultry.]]>79133142La Ragione, R.M. and M.J. Woodward,2003Bacillus subtilis spores of Salmonella enterica serotype Enteritidis and Clostridium perfringens in young chickens.]]>94245256Monteiro, S.M., J.J. Clemente, A.O. Henriques, R.J. Gomes, M.J. Carrondo and A.E. Cunha,2005Bacillus subtilis.]]>2110261031Moriarty, D.J.W.,1998Vibrio species in penaeid aquaculture ponds.]]>164351358New, M.B.,19908899143Omer, A.M.,20107124131Pandiyan, P., D. Balaraman, R. Thirunavukkarasu, E.G.J. George, K. Subaramaniyan, S. Manikkam and B. Sadayappan,201355559Rengpipat, S., S. Rukpratanporn, S. Piyatiratitivorakul and P. Menasaveta,2000Penaeus monodon) by a probiont bacterium (Bacillus S11).]]>191271288Sakai, M., T. Otubo, S. Atsuta and M. Kobayashi,1995Oncorhynchus mykiss (Walbaum), by oral administration of Clostridium butyricum bacterin.]]>18187190Sanders, M.E., L. Morelli and T.A. Tompkins,2003Bacillus, Sporolactobacillus and Brevibacillus.]]>2101110Smith, P. and S. Davey,1993Aeromonas salmonicida from fish with stress-inducible furunculosis by a fluorescent pseudomonad.]]>16521524Vaseeharan, B. and P. Ramasamy,2003Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon.]]>368387Westerdahl, A., J.C. Olsson, S. Kjelleberg, P.L. Conway,1991Scophtalmus maximus)-associated bacteria with inhibitory effects against Vibrio anguillarum.]]>5722232228