Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.2046.2049Escherichia coli MTCC 1652 Whole Cells for Bioconversion of Glycyrrhizinic acid and into 18-β Glycyrrhetinic Acid]]>AhmadM. PandaB.P. 1220131624Microbial biotransformation of Glycyrrhizinic acid (GL) into 18-β Glycyrrhetinic Acid (GA) was achieved using Escherichia coli MTCC 1652 whole cell. The E. coli whole cell was immobilized by entrapment method within calcium alginate beads using cell suspension of equal volume with sodium alginate 8%. The pH of solution, reaction volume and % of GL were optimized during the immobilization procedure and optimum pH 6.5, reaction volume of 4 mL and at 3% GL concentration for 12 h of incubation time showed highest concentration of GA (72.649 μg mL-1) with 76% bioconversion of GL to GA. Under optimized condition the immobilized cell produces 58.663 μg per mL of GA in licorice root extract containing 95.118 μg of GL per mL of the extract with 61% conversion at 12 h.]]>Amin, H.A.S., H.A. El-Menoufy, A.A. El-Mehalawy and E.S. Mostafa,2011Aspergillus terreus β-d-glucuronidase.]]>695459.Anisha, G.S. and P. Prema,2008Streptomyces griseoloalbus.]]>9933253330Barin, M., M. Otadi, F. Khorasheh and A. Kheirolomoom,2007166973.Gisbon, M.R.,1978Glycyrrhiza in old and new perspectives.]]>41348353.Greenberg, N., B. Tarakovsky, G. Yrime, S. Ulitzur and M. Sheintuch,1995coli.]]>51743756.Hennell, J.R., S. Lee, C.S. Khoo, M.J. Gray and A. Bensoussan,2008Glycyrrhiza uralensis Fisch. ExDC. (Zhi Gan Cao) root and the dried aqueous extract by LC-DAD.]]>47494500Roy, I., S. Sharma and M.N. Gupta,200486159189.Samuelsson, G.,1993pp. 127-135.pp. 127-135.Spizzizen, J., J.C. Kenney and B. Hampil,195162323329Wang, J., Q. Sun, P. Gao, J.F. Wang and C. Xu et al.,2010Aspergillus parasiticus Speare BGB.]]>46421466.Zhang, W.Y., P. Prabhu and J.K. Lee,2010Escherichia coli whole cells harboring L-arabinose isomerase for L-ribulose production.]]>33741748