Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.1991.1996KarR.K. ParvinN. LahaD. 1220131624Regulation of stomatal aperture is crucial in terrestrial
plants for controlling water loss and gaseous exchange with environment. While
much is known of signaling for stomatal opening induced by blue light and the
role of hormones, little is known about the regulation of stomatal closing in
darkness. The present study was aimed to verify their role in stomatal regulation
in darkness. Epidermal peelings from the leaves of Commelina benghalensis
were incubated in a defined medium in darkness for 1 h followed by a 1 h incubation
in different test solutions [H2O2, propyl gallate, ethrel
(ethylene), AgNO3, sodium orthovanadate, tetraethyl ammonium chloride,
CaCl2, LaCl3, separately and in combination] before stomatal
apertures were measured under the microscope. In the dark stomata remained closed
under treatments with ethylene and propyl gallate but opened widely in the presence
of H2O2 and AgNO3. The opening effect was largely
unaffected by supplementing the treatment with Na-vanadate (PM H+
ATPase inhibitor) and tetraethyl ammonium chloride (K+-channel inhibitor)
except that opening was significantly inhibited by the latter in presence of
H2O2. On the other hand, H2O2 could
not override the closing effect of ethylene at any concentrations while a marginal
opening of stomata was found when Ag NO3 treatment was given together
with propyl gallate. CaCl2 treatment opened stomata in the darkness
while LaCl3 maintained stomata closed. A combination of LaCl3
and propyl gallate strongly promoted stomatal opening. A probable action of
ethylene in closing stomata of Commelina benghalensis in dark has been
proposed.]]>Allen, G.J., P.C. Sarah, C.L. Harrington, K. Schumacher and T. Hoffmann et al.,200141110531057An, Z., W. Jing, Y. Liu and W. Zhang,2008Vicia faba.]]>59815825Desikan, R., M.K. Cheung, A. Clarke, S. Golding and M. Sagi et al.,2004Pisum sativum.]]>31913920Desikan, R., K. Last, R. Harrett-Williams, C. Tagliavia and K. Harter et al.,200647907916Fan, L.M., Z.X. Zhao and S.M. Assmann,20047537546Jannat, R., M. Uraji, M. Morofuji, M.M. Islam and R.E. Bloom et al.,2011Arabidopsis guard cells.]]>16819191926Kwak, J., I.C. Mori, Z.M. Pei, N. Leonhardt and M.A. Torres et al.,2003Arabidopsis.]]>2226232633Laha, D., N. Parvin and R.K. Kar,2009Commelina benghalensis.]]>2009Lee, K.H., H.L. Piao, H.Y. Kim, S.M. Choi and F. Jian et al.,200612611091120Pandey, S., W. Zhang and S.M. Assmann,200758123252336Parvathi, K. and A.S. Raghavendra,1997Commelina benghalensis is maximal at low calcium.]]>101861864Pei, Z.M., Y. Murata, G. Benning, S. Thomine and B. Klusener et al.,2000406731734Roelfsema, M.R.G. and R. Hedrich,2005167665691Rogers, C., P.J.H. Sharpe and R.D. Powell,1980Vicia faba in CO2-free air: Effect of temperature on stomatal aperture and potassium accumulation.]]>6510361038Schroeder, J.I., G.J. Allen, V. Hugouvieux, J.M. Kwak and D. Waner,200152627658She, X.P., A.X. Huang, J. Li and X.Z. Han,2010Vicia faba.]]>140258268Song, X.G., X.P. She, J. Wang and Y.C. Sun,2011Vicia faba.]]>38767777Talbott, L.D. and E. Zeiger,199611110511057Talbott, L.D. and E. Zeiger,199849329337Tanaka, Y., T. Sano, M. Tamaoki, N. Nakajima, N. Kondo and S. Hasezawa,2005Arabidopsis.]]>13823372343Tanaka, Y., T. Sano, M. Tamaoki, N. Nakajima, N. Kondo and S. Hasezawa,2006Arabidopsis.]]>5722592266Zeiger, E.,198334441475Zhang, X., H. Wang, A. Takemiya, C.P. Song, T. Kinoshita and K. Shimazaki,2004+ pumping by abscisic acid through hydrogen peroxide-induced dephosphorylation of the plasma membrane H+-ATPase in guard cell protoplasts.]]>13641504158