Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2012.333.340Podontia quatuordecimpunctata (Coleoptera: Chrysomelidae)]]>UddinMohammad Mosleh Hasan ChowdhuryMd. Mahmudul MojumderSuman SikdarDwaipayan 72012157Endo-β-1, 4-glucanase (EC 3.2.1.4) activity was measured in the gut fluid of phytophagous insect Podontia quatuordecimpunctata Linnaeus (Coleoptera: Chrysomelidae) in different days of development. The eight day-old larva showed maximum activity with 1.73 U mg-1 of protein, which was confirmed by gel zymography. In zymogram, using Carboxymethyl Cellulose (CMC) as substrate, four distinct cellulolytic protein bands were detected in leaf borer gut fluids through out of its development. The optimum temperature and pH were 60°C and 5.0, respectively. This endo-β-1,4-glucanase showed maximum stability at 20-45°C with ~ 20% remaining activity. Zymography also showed complete loss of endo-β-1,4 glucanase activity at 55°C. 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