Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2011.226.231Saccharopolyspora sp. PNR11]]>KhucharoenphaisanK. SinmaK. 32011143The strain PNR11 was isolated from gut of termite during the screening for uric acid degrading actinomyces. This strain was able to produce an intracellular uricase when cultured in fermentation medium containing uric acid as nitrogen source. Base on its morphological characters and 16S rDNA sequence analysis, this strain belong to the genus Saccharopolyspora. This is the first report of uricase produced from the genus Saccharopolyspora. The aim of this study was to investigate the effects of different factors on uricase production by new source of Saccharopolyspora. Saccharopolyspora sp. PNR11 was cultured in production medium in order to determine the best cultivation period. The result showed that the time period required for maximum enzyme production was 24 h on a rotary shaker operating at 180 rpm. Optimized composition of the production medium consisted of 1% yeast extract, 1% maltose, 0.1% K2HPO4, 0.05% MgSO4 7H2O, 0.05% NaCl and 1% uric acid. The optimum pH and temperature for uricase production in the optimized medium were pH 7.0 and 30°C, respectively. When the strain was cultured at optimized condition, the uricase activity reached to 216 mU mL-1 in confidential level of 95%. The crude enzyme had an optimum temperature of uricase was 37°C and it was stable up to 30°C at pH 8.5. The optimum pH of uricase was 8.5 and was stable in range of pH 7.0-10.0 at 4°C. This strain might be considered as a candidate source for uricase production in the further studies. Present finding could be fulfill the information of uricase produce from actinomycetes.]]>Atalla, M.M., M.M. Farag, R.H. Eman, M.S. Abd-El-Lataif and E.A. Nehad,2009Gliomastix gueg.]]>54550Bertrand, K.E., N. Mathieu, G. Inocent and F.K. Honore,2008Plasmodium falciparum malaria in Douala.]]>1116461649Bomalaski, J.S. and M.A. Clark,20046240247Bongaerts, G.P.A., J. Uizetter, R. Brouns and G.D. Vogels,1978Bacillus fastidiosus properties and regulation of synthesis.]]>527348358Frank, W. and G.E. Hahn,1955Pseudomonas aerogenosa (B. pyocyaneum).]]>30190106Gochman, N. and M.J. Schmitz,19711711541159Haidari, F., M.R. Rashidi, S.A. Keshavarz, S.A. Mahboob, M.R. Eshraghian and M.M. Shahi,20081117791784Hunag, S. and T. Wu,2004Bacillus subtilis uricase gene following StEP mutagenesis.]]>271517523Ishikawa, J., A. Yamashita, Y. Mikami, Y. Hoshino and H. Kurita et al.,2004Nocardia farcinica IFM 10152.]]>1011492514930Jianguo, L., L. Gaoxiang, L. Hong and Z. Xiukai,1994Candida sp. and its application in uric acid analysis in serum.]]>475763Kai, L., X.H. Ma, X.L. Zhou, X.M. Jia, X. Li and K.P. Guo,2008Microbacterium sp. strain ZZJ4-1.]]>24401406Khucharoenphaisan, K. and K. Sinma,20102010pp: 336338Lotfy, W.A.,2008Bacillus thermocatenulatus strain.]]>99699702Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Nobutoshi, K., S. Keisuke, M. Takao, T. Masaki, K. Hitoshi and T. Kazue,20001612031205Saeed, H.M., Y.R. Abdel-Fattah, Y.M. Gohar and M.A. Elbaz,2004Pseudomonas aeruginosa urate oxidase enzyme.]]>534552Schiavon, O., P. Caliceti, P. Ferruti and F.M. Veronese,200055264269Sin, I.L.,1975Pseudomonas acidovorance.]]>4101220Yazdi, M.T., G. Zarrini, E. Mohit, M.A. Faramarzi, N. Setayesh, N. Sedighi and F.A. Mohseni,2006Mucor hiemalis: A new source for uricase production.]]>22325330Yokoyama, S., A. Ogawa and A. Obayashi,1988Candida utilis cells by use of reducing agent plus surfactant.]]>105255Zhou, X., X. Ma, G. Sun, X. Li and K. Guo,20054037493753