Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2009.189.191Pisolithus tinctorius Induced by the Plant Metabolites]]>Herrera-MartinezAseneth Ruiz-MedranoRoberto ValdesMaria Xoconostle-CazaresBeatriz 22009122The aim of this study was to test the effect of acetosyringone
(AS) on the accumulation of the histidine kinase coding mRNA, using in
vitro propagated Pisolithus tinctorius. In the precontact phase
of the ectomycorrhizal symbiosis, it is hypothesized that the plants releases
chemicals, which are sensed by the fungal mycelium and in turn trigger
the start of the symbiosis. The nature of such molecules is largely unknown;
however, plant metabolite and their structural analogues have been widely
used to induce infection in different microbe-plant interactions. A histidine
kinase in Agrobacterium tumefaciens was activated by AS in the
first step during the infection of dicotyledonous plants. A conserved
gene fragment from the ectomycorrhizal fungus Pisolithus tinctorius
was cloned using degenerate primers of conserved regions characteristic
of the histidine kinase gene family. Then, the accumulation of this transcript
in fungi incubated with AS was analyzed. RT-PCR and in situ hybridization
suggest that this mRNA is synthesized in the presence of this plant analog
in AS-treated mycelia. The findings presented here suggest a role of a
histidine kinase involved in the early stages of ectomycorrhizal differentiation.]]>Akiyama, K., K. Matsuzaki and H. Hayashi,2005435824827Barker, S.J., D. Tagu and G. Delp, 199811612011207Barker, S.J. and D. Tagu, 200019144154Brencic, A. and S.C. Winans,200569155194Calera, J.A., D. Herman and R. Calderone, 2000YPD1, a gene of Candida albicans which encodes a two-component phosphohistidine intermediate protein.]]>1610531059Grefen, C. and K. Harter, 2004219733742Jones, C.A., S.E. Greer-Phillips and K.A. Borkovich,2007Neurospora crassa.]]>1821232136Klumpp, S. and J. Krieglstein, 2002410671071Martin, F., A. Aerts, D. Ahren, A. Brun and E.G.J. Danchin et al.,2008Laccaria bicolor provides insights into mycorrhizal symbiosis.]]>4528892Marx, D.H., 1977Pisolithus tinctorius.]]>23217223Podila, G.K., J. Zheng, S. Balasubramanian, S. Sundaram and S. Hiremath,2002Laccaria bicolor and red pine.]]>244117128Rodriguez-Tovar, A.V., R. Ruiz-Medrano, A. Herrera-Martínez, B.E. Barrera-Figueroa and M.E. Hidalgo-Lara et al., 2005Pisolithus tinctorius.]]>634554Ruiz-Medrano, R., B. Xoconostle-Cázares and W.J. Lucas,1999CmNACP-1 mRNA: Implications for supracellular regulation in plants.]]>12645054519Sambrook, J. and D.W. Russell, 20013rd Edn.,Smith, S.E. and D.J. Read,19972nd Edn.,Pages: 605Pages: 605Valdés, M., 198564885888Lee, Y.W., S. Jin, W.S. Sim and E.W. Nester,1996Agrobacterium: Genetic evidence for direct recognition of phenolic inducers by the VirA protein.]]>1798388