Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2009.1462.1467AlalayahW. M.KalilM. S.KadhumA.A. H.JahimJ. M.JaaparS.Z. S.AlaujN. M.1220091222A two-stage fermentation process consisting of dark and photo-fermentation periods was carried out in a batch reactor. In the first stage, glucose was fermented in the dark stage using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564; CSN1-4) to produce acetate, CO2 and H2. The acetate produced in the first stage is fermented to H2 and CO2 by Rhodobacter sphaeroides NCIMB 8253 for further hydrogen production in the second, illuminated stage. The yield of hydrogen in the first stage was about 3.10 mol H2 (mol glucose)-1 at a glucose concentration of 10 g L-1, pH 6±0.2 and 37°C and the second stage yield was about 1.10-1.25 mol H2 (mol acetic acid)-1 at pH 6.8±0.2 and 32°C, without removal of the Clostridium CSN1-4. The overall yield of hydrogen in the two-stage process, with glucose as the main substrate was higher than single-stage fermentation.]]>Alalayah, W.M., M.S. Kalil, A.H. Kadhum, J.M. Jahim and N.M. Alaug,2008Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564).]]>3373927396Alalayah, W.M., M.S. Kalil, A.H. Kadhum, J.M. Jahim and N.M. Alaug,2009C. saccharoperbutylacetonicum N1-4(ATCC 1356 4).]]>58086Asada, Y., M. Tokumoto, Y. Aihara, M. Oku, K. Ishmi and T. Wakayama,2006Lactobacillus and a photosynthetic bacterium Rhodobacter sphaeroides RV.]]>3115091513Hipolito, C.N., E. Crabbe, C.M. Badillo, O.C. Zarrabal and M.A.M. Mora et al.,2008Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564).]]>16632638Das, D. and T.N. Veziroglu,2001261328Fabiano, B. and P. Perego,2002Enterobacter aerogenes.]]>27149156Han, S.K. and S.H. Shin,200429569577Hawkes, F.R., R. Dinsdale, D.L. Hawkes and I. Hussy,20022713391347Kalil, M.S., P.W. Kit, W.M.W. Yusoff, Y. Sadazo and R.A. Rahman,2003612731275Kapdan, I.K. and F. Kargi,200638569582Nath, K., A. Kumar and D. Das,2006Enterobacter cloacae DM11.]]>52525535Kotay, S.M. and D. Das,200833258263Lay, J.J.,200174280287Liu, H., T. Zhang and H.H.P. Fang,200325365369Liu, G. and J. Shen,200498251256Manish, S. and R. Banerjee,200833279286Miller, G.L.,195931426428Nath, K., A. Kumar and D. Das,2005Enterobacter cloacae DM11.]]>68533541Nath, K. and D. Das,200465520529Ni, M., D.Y.C. Leung, M.K.H. Leung and K. Sumathy,200687461472Oh, Y.K., E.H. Seol, M.S. Kim and S. Park,2004Rhodopseudomonas palustris P4.]]>2911151121Oh, Y.K., E.H. Seol, J.R. Kim and S. Park,2003Citrobacter sp. Y19.]]>2813531359Shi, X.Y. and H.Q. Yu,2004Rhodopseudomonas capsulate.]]>117143154Niel, E.W.J., P.A.M. Claassen and A.J.M. Stam,2003Caldicellulosiruptor saccharolyticus.]]>81255262Wooshin, P., H.H. Seung, E.O. Sang, E.L. Bruce and K. Ins,20063944164420Yokoi, H., A. Saitsu, H. Uchida, J. Hirose, S. Hayashi and Y. Takasaki,2001915863Yokoi, H., R. Maki, J. Hirose and S. Hayashi,200222389395