Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2009.134.139Bubalus bubalis) Bulls]]>Alavi-ShoushtariS.M. RezaiS. Asri AnsariM.H. Kh. KhakiA. 22009122In order to determine zinc and catalase content of seminal
plasma in the buffalo and to study their associations with the semen characteristics,
54 semen samples were collected from 10 buffalo bulls; semen volume and
sperm concentration, gross and progressive motility and viability were
evaluated, seminal plasma was then harvested by centrifugation and its
zinc content was estimated by atomic absorption spectrophotometer and
its catalase activity determined by using a commercial kit. The zinc content
of the seminal plasma (Mean ± SEM) was recorded as 154.40 ±
1.74 mg L-1, while, the mean catalase value was 32.00 ±
0.42 U mL-1. The mean zinc values was highly correlated with
sperm progressive motility and viability and with catalase values (p =
0.000 for all) and also was associated with gross motility (p = 0.020)
and negatively with abnormal morphology (p = 0.049). The catalase values
were highly associated with sperm progressive motility, viability and
zinc content (p = 0.000 for all) and was associated with sperm gross motility
(p = 0.024). For further clarification of these correlations, the samples
were categorized in three groups of excellent (Ex, > 90% motile, n
= 33), good (Go, 80-89% motile, n = 15) and moderate (Mo, < 79% motile,
n = 6) according to their percentage of sperm motility. The mean progressive
motility in Ex group was 92.54 ± 0.51%, in Go group was 81.66 ±
0.62% and in Mo group was 71.66 ± 1.05%. The mean zinc and catalase
values were recorded as 161.07 ± 1.63 mg L-1 and 33.41
± 0.34 U mL-1 in Ex, 146.70 ± 1.91 mg L-1
and 31.01 ± 0.67 in Go and 136.42 ± 4.97 mg L-1
and 26.51 ± 0.87 U mL-1 in Mo groups. The mean zinc
value in Ex group was highly associated with sperm motility, viability
and catalase values, in Go group was associated with catalase values and
highly associated with sperm abnormal morphology and in Mo group it was
highly associations with catalase values only. The mean catalase value
in Ex group, was highly associated with sperm motility and viability,
in Go group was associated with zinc content and in Mo groups was highly
associated with the zinc content. These results show that seminal plasma
zinc and catalase content are correlated with semen characteristics and
synergistically act to preserve motility and viability of the spermatozoa
after ejaculation.]]>Ahotupa, M. and I. Huhtaniemi,19924611141118Ax, R.L., M.A. Dally, R.W. Lenz, C.C. Love, D.D. Varner, B. Hafez and M.E. Bellin,20002000pp: 365-375pp: 365-375Barth, A.D.,20072007pp: 235-239pp: 235-239Baumber, J., K. Sabeur, A. Vo and B.A. Ball,2003oxygen species promote tyrosine phosphorylation and capacitation in equine spermatozoa.]]>6012391247Bilodeau, J.F., S. Blanchette, N. Comier and M.A. Sirard,2002oxygen species-mediated loss of bovine sperm motility in egg yolk Tris extender: Protection by pyruvate, metal chelator and bovine liver or oviductal fluid catalase.]]>5711051122Cordoba, M., N. Mora and M.T. Beconi,200665882892De Graaf, S.P., G. Evans, L. Gillan, M.M.P. Guerra, W.M.C. Maxwell and J.K. O’Brien,2007in vitro quality of stored and non-stored ram spermatozoa.]]>67217227Turner, T.T. and J.J. Lysiak,200829488498Eickhoff, R., C. Baldauf, H.W. Koyro, G. Wennemuth and Y. Suga et al.,200410605611Strzezek, J., E. Hopfer and A. Zaborniak,198713133142Holtz, W. and R.H. Foote,197818286292Kendall, N.R., S. McMullen, A. Green and R.G. Rodway,200062277283Kumar, N., R.P. Verma, L.P. Singh, V.P. Varshney and R.S. Dass,2006Bos indicus × Bos taurus) bulls.]]>46663675Lapointe, S., R. Sullivan and M.A. Sirard,199858747753Lapointe, S., J.F. Bilodeau, D. Lemieux, E. Asselin, M.A. Fortier and M.A. Sirard,2000in vitro protect bovine sperm from hydrogen peroxide.]]>54355369Lin, Y.C., T.C. Chang, Y.J. Tseng, Y.L. Lin, F.J. Huang, F.T. Kung and S.Y. Chang,200023260266Lindemann, C.B., J.A. O'Brien and F.J. Giblin,198838114120Marti, E., L. Mara, J.I. Marti, T. Muino-Blanco and J.A. Cebrian-Perez,20076714461454Massanyi, P., J. Trandzik, P. Nad, R. Toman, M. Skalicka and B. Korenekova,20035101104Massanyi, P., J. Trandzik, P. Nad, B. Korenekova, M. Skalicka et al.,20033826432651Massanyi, P., J. Trandzik, P. Nad, M. Skalicka and B. Korenekova et al.,20054010971105Zubkova, E.V. and B. Robaire,20047110021008Sanocka, D., R. Meisel, P. Jedrzejczak and M.K. Kurpisz,199617449454Sansone, G., M.J.F. Nastri and A. Fabbrocini,2000Bubalus bubalis) semen.]]>625576Verberckmoes, S., A. Van Soom, J. Dewulf and A. de Kruif,200563912922Vallee, B.L. and K.H. Falchuk,19937379118Stoltenberg, M., M.B. Sorensen, G. Danscher, S. Juhl, A. Andreasen and E. Ernst,19973763767Strzezek, J. and E. Hopfer,198713117131