Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.2629.2633 Brassica napus Using Random Amplified Polymorphic DNA Markers]]>FazeliE. ShahriariF. SamizadehH. BagheriA. FarsiM. 1220081123In current research, genetic relationships among rapeseed genotypes from several geographical origins including France, Canada, Germany, Iran, Hungary, Denmark, Australia and America were evaluated using RAPD markers. Among generated 86 bands, 80 different polymorphic bands were obtained using 9 random primers. Diversity Index (DI) or Polymorphism Information Content (PIC) was varied from 0.29 to 0.48, showed a relatively high potential of primers among studied genotypes. Dice similarity coefficient between genotypes was calculated using Nei and Li formula. Maximum (0.91) and minimum (0.42) similarity coefficients were observed between Bristol and Amber genotypes, consul and express, respectively. Cluster analysis based on dice similarity coefficient was also carried out. Base on the cluster analysis, genotypes were grouped into five main clusters. Results showed that genotypes with same geographical origin were genetically different. Therefore, geographical origins of genotypes cannot be used as a base to cross parent to obtain high heterosis and it must be carried out by exact genetic studies. Results confirmed that RAPD is a simple, cheap and fast method for evaluation of genetic diversity of Brassica napus.]]>Botstein, D., R.L. With, M. Skolnick and R.W. Davis,198032314331Cruz, V.M.V., R. Luhman, L.F. Marek, C.L. Rife, R.C. Shoemaker, E.C. Brummer and C.A.C. Gardner,2007Brassica napus L. germplasm.]]>1534357Davis, T.M., H. Yu, K.M. Haigis and P.J. McGowan,199591582588Dellaporta, S.L., J. Wood and J.B. Hicks,198311921Demeke, T., R.P. Adums and R. Chibbar,1992Brassica.]]>84990994Diers, B.W. and T.C. Osborn,1994Brassica napus germ plasm based on restriction fragment length polymorphisms.]]>88662668FAO,20052005FAO,20072007Hallden, C., N.O. Nilsson, I.M. Rading and T. Sall,1994Brassica napus breeding lines.]]>88123128Heun, M. and T. Helentjaris,199385691698Hu, J., G. Li, D. Struss and C.F. Quiros,1999Brassica napus.]]>118145150Jordan, D.R., Y.Z. Tao, I.D. Godwin, R.G. Henzell, M. Cooper and C.L. McIntyre,199810217Kresovich, S., J.G.K. Williams, J.R. McFerson, E.J. Routman and B.A. Schaal,1992Brassica oleracea L. via a random amplified polymorphic DNA assay.]]>85190196Mailer, R.J., N. Wratten and M. Vonarx,199737793800Nei, M. and W.H. Li,19797652695273Quiros, C.F., J. Hu, P. This, A.M. Chevre and M. Delseny,1991Brassica.]]>82627632Rajcan, I., K.L. Kasha, L.S. Kott and W.D. Beversdorf,1999Brassica napus L.).]]>105173181Shengwu, H.U., J. Ovesna, L. Kucera, V. Kucera and M. Vyvadilova,2003Brassica napus germplasm from China and Europe assessed by RAPD markers.]]>49106113Tanhuanpaa, P.K., J.P. Vilkkim and H.J. Vilkki,1995Brassica.napus L.).]]>38414416Thormann, C., M. Ferreira, L. Camargo, J. Tivang and T. Osborn,199488973980Welsh, J., C. Peterson and M. McClelland,199119303306