Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.2423.2427ZhangX.Y. ZhouM.L. ZhangX.H. WuD.J. 1220081120To evaluate the genetic polymorphisms and to search
for available molecular markers for Liangshan semi-wool sheep, 15 microsatellite
markers of 187 samples were amplified by multiplex PCR. A total of 133
alleles were detected, with the number of alleles ranging from 6 (BM2830)
to 15 (McM130), giving a mean No. of 8.87 alleles per locus. The total
and mean effective allele No. were 64.29 and 4.29, respectively. The observed
heterozygosity and expected heterozygosity were from 0.4486 (McM130) to
0.8877 (BMS1678) and 0.5704 (BMS0887) to 0.8373 (McM130), respectively.
Mean observed heterozygosity and mean expected heterozygosity were 0.672
and 0.7536. Polymorphism information content values were from 0.5202 (BMS0887)
to 0.8183 (McM130) and mean polymorphism information content of the 15
microsatellite loci was 0.7184. All 15 microsatellite loci were highly
polymorphic, which showed that there were rich genetic polymorphisms at
these detected microsatellite loci in Liangshan semi-wool sheep. Comparison
of allele distributions among loci did not reveal consistent shapes. Distributions
were centralized in some cases, whereas in others some kind of skewness
was evident. Complex evolution at these loci is an important factor in
the irregularity of microsatellite distributions.]]>Alvarez, I., L.J. Royo, I. Fernandez, J.P. Gutierrez, E. Gomez and F. Goyache,20048222462252Alvarez, I., L.J. Royo, J.P. Gutierrez, I. Fernandez, J.J. Arranz and F. Goyache,20081158088Arora, R. and S. Bhatia,200454227230Arranz, J.J., Y. Bayon and F. San Primitivo,199829435440Botstein, D., R.L. White, M. Skolnick and R.W. Davis,198032314331Calvo, J.H., J.A. Bouzada, J.J. Jurado and M. Serrano,200664116125Forbes, S.H., J.T. Hogg, F.C. Buchanan, A.M. Crawford and F.W. Allendorf,19951211061113Gao, A.B. and D.J. Wu,2005278590Goldstein, D.B. and D.D. Pollock,199788335342Kimura, M. and J.F. Crow,196449725738Levene, H.,1949209194Sambrook, J., E.F. Fritsch and T.A. Maniatis,19892nd Edn.,Pages: 397Pages: 397Sodhi, M., M. Mukesh and S. Bhatia,200665185192Stahlberger-Saitbekova, J., G. Schlapfer Dolf and C. Gaillard,2001118379387Sun, W., H. Chang, D. Ji, X. Liao, L. Du, S. Lu and T. Kenji,20073410011009Wang, G.F. and D.J. Wu,20062815051512Wrights, S.,1978Vol. 4.,Yeh, F.C., R. Yang and T. Boyle,1999Version 1.31,Zhang, M.Y. and D.J. Wu,200527575578Zhou, M.L., D.J. Wu and X.Y. Zhang,20072914751482