Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.2346.2350Trichoderma spp. on Tea Seedlings White Foot and Root Rot (Sclerotium rolfsii Sacc.) in vitro Condition]]>ShaiganS. SerajiA. MoghaddamS.A.M. 1220081119The aim of this two years research work is to recognize
bio control agents and its antagonistic efficiency on Sclerotium rolfsii
in vitro condition. Sclerotium rolfsii Sacc. is a causal agent
of white foot and root rot disease in many plants. This fungi causing
damage in tea nurseries of tea cultivated countries especially in Iran.
Due to importance this disease in tea nurseries of Iran and impossibility
using of chemical control against this damaging agent In this research,
five species of Trichoderma viride, Trichoderma harzianum,
Trichoderma hamatum, Trichoderma longibrachiatum and
Trichoderma parceramosum as antagonist fungi against Sclerotium
rolfsii were collected and identified from tea nurseries and seedlings
in Iran. Then efficiency of these bio control agent against Sclerotium
rolfsii were investigated in vitro condition. Results showed
that these species with different mechanism such as lysis of sclerotia,
inhibited mycelial growth of Sclerotium rolfsii with volatile metabolites
producing and parasitized the hyphal trends of disease agent were showed
its antagonistic effects against causal agent of white foot and root rot
in tea seedling. Trichoderma viride, Trichoderma harzianum and
Trichoderma hamatum after 30 days destructed and lysis the sclerotia
98.5, 86.5 and 85%, respectively. Producing of volatile metabolites after
72 h reciprocal growth of Trichoderma viride, Trichoderma harzianum
and Trichoderma longibrachiatum till 60.8, 54.8 and 54.4% prevented
mycelium growth of Sclerotium rolfsii, respectively. All identified
species caused parasitize (to hyphal contact method) pathogen fungal hypha
that the first three species showed more parasite effect.]]>Alexopoulos, C.J., C.W. Mims and M.M. Blackwell,19964th Edn.,Pages: 868Pages: 868Barnett, H.L. and B.B. Hunter,19723rd Edn.,Pages: 331Pages: 331Chet, I.,1987Trichoderma: Application, Mode of Action and Potential as a Bio Control Agent of Soil Borne Plant Pathogenic Fungi.]]>1987pp: 137-160pp: 137-160Dennis, C. and J. Webster,1971Trichoderma: I. Production of non-volatile antibiotics.]]>572539Dennis, C. and J. Webster,1971Trichoderma: II. Production of volatile antibiotics.]]>574148Dennis, C. and J. Webster,1971Trichoderma: III. Hyphal interaction.]]>57363369Elad, Y., A. Kalfon and I. Chet,1982Rhizoctonia solani in cotton by seed coating with Trichoderma spp. spores.]]>66279281Ershad, G.,19952097577Harman, G.E., I. Chet and R. Baker,1980Trichoderma hamatum effects on seed and seedling disease induced in radish and pea by Pythium spp. or Rihizoctonia solani.]]>7011671172Khodaparast, S.A. and G.A. Hedjaroud,199632233243Okhovvat, M. and D. Vakili,19981st Edn.,pp: 236-239pp: 236-239Papavizas, G.C.,1985Trichoderma and Gliocladium: Biology, ecology and potential for biocontrol.]]>232354Punja, Z.K.,1985Sclerotium rolfsh.]]>2397127Rifai, M.A.,1969Trichoderma: The Commonwealth Mycological Institute.]]>1155556Santos, I.D. and W. Bettiol,2003Sclerotium rolfsii.]]>2210931097Tsahouridou, P.C. and C.C. Thanassoulopoulos,2002Trichoderma koningii as a potential parasite of sclerotia of Sclerotium rolfsii.]]>22289295Tsahouridou, P.C. and C.C. Thanassoulopoulos,2002Trichoderma koningii in the tomato rhizosphere and the suppression of damping off by Sclerotium rolfsii.]]>34767776Zeppa, G., G. Allegron, M. Barbeni and P.A. Gurda,1991Trichoderma viride.]]>7047354735