Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.2282.2285MohsenzadehM. BahrainipourA. 1220081118 The aim of this study was to study performance of Yoghurt
Culture Test (YCT) in the detection of antimicrobial residues in milk.
For this purpose, the sensitivity of YCT for 15 antibiotics were determined.
For each drug, 8 concentrations were tested. The detection limits of YCT
at 2.5 h and 4 h incubation were determined (μg kg-1):
15 and 37.5, penicillin G; 4 and 5, ampicillin; 5 and 7.5, amoxycillin;
100 and 200, cephalexin; 80 and 100, cefazoline; 100 and 200, oxytetracycline;
500 and 100, chlortetracycline; 100 and 200, tetracycline; 150 and 200,
doxycycline; 200 and 400, sulphadimidine; 500 and 1000, gentamycin; 1000
and 1500, spectinomycin; 400 and 500, erythromycin; 50 and 100, tylosin;
5000 and 10000, chloramphenicol. The YCT detection limits at 2.5 h incubation
for ampicillin, cephalexin, tetracycline, oxytetracycline and tylosin
are similar to those obtained as Maximum Residue Limit (MRL) according
to Regulation 2377/90 EEC as set out by the European Union. In addition
the detection limits of YCT for some antibiotics were lower than some
of microbial inhibitor test.]]>Althaus, R.L., A. Torrer, A. Montero and M.P. Molina,200386457463Aurelli, P., A. Ferrini and V. Mannoni,19967165168Currie, D., L. Lynas, G. Kennedy and J. McCaughey,199815651660Dewdney, J.M., L. Maes, J. P. Raynaud, F. Blanc and J.P. Scheid et al.,199129477483Gaudin, V. and P. Maris,2001137786Kolosova, A.Y., J.V. Samsonova and A.M. Egorov,200012115125Molina, M.P., R.L. Althaus, A. Molina and N. Fernandez,200313821826Montero, A., R.L. Althaus, A. Molina, I. Berruga and M.P. Molina,200557229237Nouws, J., H. Van Egmond, I. Smulders, G. Loeffen and J. Schouten et al.,199998590Nouws, J., G. Loeffen, J. Schouten, H. van Egmond, H. Keukens and H. Stegeman,19988123412345Reichmuth, J., G. Suhren and R. Beukers,199752691694Robinson, R.K. and A.Y. Tamime,20022002Scannella, D., P. Neaves, K. Keedy and C. Bell,199779396Suhren, V.G.,2002543571Suhren, G. and W. Heeschen,199348259263Suhren, G. and H.G. Walte,200358536540Suhren, G. and K. Knappstein,199812327972801Yamani, M.I., L.M.A. Al-Kurdi, M.S.Y. Haddadin and R.K. Robinson,1999103539