Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.1436.1442Vitis vinifera]]>Rigi NazhadNafiseh SoloukiMahmood 1120081111In the present study, three DNA extraction procedures were examined
to determine which might yield DNA from Grape leaves suitable for molecular
analysis for RAPD, SSR. AFLP and etc analysis. The three methods examined
were: the miniprep procedure and the modified CTAB for difficult species
and protocol CTAB. Only the modified CTAB method consistently yielded
DNA suitable for Polymerase Chain Reaction (PCR) amplification, regardless
of plant growing conditions or leaf age. The quality and quantity of extracted
genomic DNA gained from these methods are deliberated by means UV biophotometer,
electrophoresis in 1.2% agarose gel and PCR. In this regard, application
chosen for young and mature leaves, the most value of qualified DNA, is
extracted from fully expanded leave when PVP was added to the extraction
buffer. This same procedure also yielded PCR-amplifiable DNA from various
other perennial, woody species and from other fruit species such as apple
(Malus domestica), cherry (Prunus avium), peach (Prunuspersica),
plum (Prunus domestica). DNA yield from this procedure is high
(up to 1 mg g-1 of leaf tissue). DNA is completely digestible
with restriction endonucleases and amplifiable in the Polymerase Chain
Reaction (PCR).]]>Adam-Blondon, A.F., C. Roux, D. Claux, G. Butterlin, D. Merdinoglu and P. This2004Vitis vinifera genome: A tool for grape genetics.]]>10910171027Akkurt, M., L. Welter, E. Maul, R. Topfer and E. Zyprian,2007Vitis vinifera L. and Vitis sp.).]]>19103111Alaey, M., R. Naderi, A. Vezvaei, A. Khalichi and A. Salami,2005Cyclamen persicum Mill.]]>7882884Arumuganathan, K. and E.D. Earle,19919208218Baker, S.S., C.L. Rugh and J.C. Kamalay,19909268272Baribault, T.J., K.G.M. Skene, P.A. Cain and N.S. Scott,19904110451049Bushra, C., A. Yasmin, T. Husnain and S. Riazuaain,199917280280Collins, G.G. and R.H. Symons,199210233235Couch, J.A. and P.J. Fritz,19908812Dellaporta, S.L., J. Wood and J.B. Hicks,198311921Deragon, J.M. and B.S. Landry,19921175180Di Gaspero, G., G. Cipriani, A.F. Adam-Blondon and R. Testolin,200711412491263Do, N. and R.P. Adams,199110162166Doyle, J.J. and J.L. Doyle,1987191115Doyle, J.J.,1990121315Draper, J. and R. Scott,19881988pp: 199-236.pp: 199-236.Fang, G., S. Hammar and R. Grumet,1992135256Harborne, J.B.,19631963pp: 13-36.pp: 13-36.Hebert, D., J.R. Kikkert, F.D. Smith and B.I. Reisch,199312585589Howland, D.E., R.P. Oliver and A.J. Davy,19919340344Katterman, F.R.H. and V.I. Shattuck,1983Gossypium species that contain large amounts of phenolic terpenoids and tannins.]]>13347359Kikkert, J.R., M.J. Striem, J.R. Vidal, P.G. Wallace, J. Barnard and B.I. Reisch,2005Vitis sp.) genotypes.]]>41232239Lodhi, M.A., G.N. Ye, N.F. Weeden and B.I. Reisch,1994Vitis species. ]]>12613Lodhi, M.A. and B.I. Reisch,1995Vitis species, cultivars and other genera of the Vitaceae.]]>901116Maliyakal, E.J.,19922023812381Manning, K.,19911954550McGoven, P.E.,20031st Edn.,Murashige, T. and F. Skoog,196215473497Murray, M.G. and W.F. Thompson,1980843214326Newbury, H.J. and J.V. Possingham,197760543547Porebski, S., L.G. Bailey and B.R. Baum,199715815Rogers, S.O. and A.J. Bendich,198556976Rogers, S.O.,19941994pp: 47-67.pp: 47-67.Sambrook, J., E.F. Fritsch and T.A. Maniatis,19892nd Edn.,Pages: 397Pages: 397Solouki, M., N.R. Nazhad, R. Vignani, B.A. Siahsar, H. Kamaladini and A. Emamjomeh,20071019962001Southern, E.M.,197598503517Stein, D.B.,1993224153167Troggio, M., G. Malacarne, G. Coppola, C. Segala and D.A. Cartwright et al.,2007Vitis vinifera L.) Anchoring pinot noir bacterial artificial chromosome contigs.]]>17626372650Varadarajan, C.S. and C.S. Prakash,19919612Wang, H., M.Qi and A.J. Cutler,19932141534154Wang, Q., P. Li, U. Hanania, N. Sahar and M. Mawassi et al.,2005Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions.]]>168565571Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey,19901865316535Zhang, J., M.B. McDonald and P.M. Sweeney,1997Peturia and Cyclamon seed using random amplified polymorphic DNA markers.]]>32246247