Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.383.387PashmiMorteza QanbariSaber Ali GhorashiSeyed SalehiAbdolreza 32007103Major Histocompatibility Complex (MHC) class II locus DRB3 was investigated
by PCR based restriction fragment length polymorphism (PCR-RFLP) assay.
A total of 262 Holstein cows participating in the national recording system
were sampled from 10 herds. A two-step polymerase chain reaction was carried
out in order to amplify a 284 base-pair fragment of exon 2 of the target
gene. Second PCR products were treated with three restriction endonucleas
enzymes RsaI, BstYI and HaeIII. Digested fragments
were analyzed by polyacrylamid gel electrophoresis. Twenty-eight BoLA-DRB3
alleles were identified. Identified alleles are: BoLA-DRB3.2 *3, *6, *7,
*8, *9, *10, *11, *12, *13, *14, *15, *16, 20, *21, *22, *23, *24, *25,
*26, *27, *28, *32, *36, *37, *40, *51, *iaa and *ibb. The BoLA-DRB3.2*40
allele that was observed in this study has not been reported previously.
The calculated frequencies were as follows: 2.29, 1.34, 0.19, 14.5, 0.38,
3.05, 12.21, 1.34, 2.29, 1.34, 2.48, 9.16, 0.95, 0.77, 6.68, 9.16, 17.94,
1.15, 0.57, 1.15, 0.95, 0.57, 0.38, 1.91, 0.38, 5.73, 0.19 and 0.95% respectively.
The six most frequently observed alleles (BoLA-DRB3.2 *8, *11, *16, *22,
*23 and *24) accounted for 69.65% of the alleles in these 10 herds. The
results of this study confirm the allelic distribution of six most frequent
alleles in Holstein population`s worldwide.]]>Amills, M., V. Ramiya, J. Norimine and H.A. Lewin,199817108120Da Mota., A.F., M.L. Martinez and L.L. Coutinho,2004313135Dietz, A.B., J.C. Detilleux, A.E. Freeman, D.H. Kelly, J.R. Stabel and M.E. Kehrli,199780400405Dietz, A.B., N.D. Cohen, L. Timms and M.E. Kehrli Jr.,199780406412Gelhaus, A., L. Schnittger, D. Mehlitz, R.D. Horstmann and C.G. Meyer,199526147153Gilliespie, B.E., B.M. Jayarao, H.H. Dowlen and S.P. Oliver,19998220492053Kelm, S.C., J.C. Deitilleux, A.E. Freeman, M.E. Jr. Kehrli and A.B. Dietz et al.,19978017671775Klein, J.,1986pp: 715-762pp: 715-762Klein, J. and V. Horejsi,19972nd Edn.,Ledwidge, S.A., B.A. Mallard, J.P. Gibson, G.B. Jansen and Z.H. Jiang,200132219221Lewin, H.A.,1996Park, Y.H., Y.S. Joo, J.Y. Park, J.S. Moon and S.H. Hyun et al.,200452939Pashmi, M., S.A. Ghorashi, A.R. Salehi, M. Moini and S. Javanmard et al.,200619775778Schukken, Y.H., B.A. Mallard, J.C.M. Dekkers, K.E. Leslie and M.J. Stear,199477639647Sharif, S., B.A. Mallard, B.N. Wilkie, J.M. Sargeant and H.M. Scott et al.,199829157160Sharif, S., B.A. Mallard and J.M. Sargeant,2000Staphylococcus species.]]>76231238Sommer, S.,200521616Starkenburg, G.E., I.G. Udina, G.O. Shaikhaev and H. Chester-Jones,19978034113411Takeshima, S., Y. Nakai, M. Ohta and Y. Aida,20028516301632Takeshima, S., N. Saitou., M. Morita, H. Inoko and Y. Aida,2003316111118Van Dorp, R.T., S.W. Martin, M.M. Shoukri, J.P. Noordhuizen and J.C. Dekkers,199963185192Van Eijk, M.J.T., J.A. Stewart-Haynes and H.A. Lewin,199223483496Sharif, S., B.A. Mallard, B.N. Wilkie, J.M. Sargeant, H.M. Scott, J.C.M. Dekkers and K.E. Leslie,199829185193