Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.3738.3741NassiryMohammad RezaMoussaviAlireza HeraviAlashawkanyAbdul RaoofGhovatiShahrokh 1220071020The objective of the present investigation was to study genetic variations in the exon II of leptin gene in Iranian Golpayegani and Taleshi cows. Blood samples were collected from 76 Golpayegani and 64 Taleshi cows randomly selected from Delijan city (Esfahan province) and Talesh city (Guilan province) in Iran, respectively. Genomic DNA was extracted from 100 micro litters of blood using guanidium thiocyanate-silica gel method. A 94 bp fragment from exon II of the bovine leptin gene was amplified using the polymerase chain reaction. Digestion of PCR products with Bsp13I restriction enzyme differentiated C and T alleles. The T allele frequencies were 0.29 and 0.45 in Golpayegani and Taleshi breeds, respectively. The frequencies of the genotypes CC, CT and TT were 0.42, 0.58 and 0 in Golpayegani and 0.36, 0.36 and 0.27 in Taleshi cows. The average heterozygosities were 0.41 and 0.50 in Golpayegani and Taleshi breeds, respectively. The χ2 test did not confirm the Hardy-Weinberg equilibrium in both populations.]]>Almeida, S.E., E.A. Almeida, J.C.F. Moraes and T. Weimer,2003120106113Blache, D., R.L. Tellam, L.M. Chagas, M.A. Blackberry, P.E. Vercoe and G.B. Martin,2000165625637Boom, R., C.J. Sol, M.M. Salimans, C.L. Jansen, P.M.E. Wertheim-van Dillen and J. van der Noordaa,199028495503Buchanan, F.C., C.J. Fitzsimmons, A.G. van Kessel, T.D. Thue, D.C.W. Sim and S.M. Schmutz,200234105116Buchanan, F.C., A.G. van Kessel, C. Waldner, D.A. Christensen, B. Laarveld and S.M. Schmutz,20038631643166Choudhary, V., P. Kumar, T.K. Bhattacharya, B. Bhushan and A. Sharma,2005Bos indicus and Bos taurus cattle.]]>28740742Ehrhardt, R.A., R.M. Slepetis, J. Siegal-Willott, M.E. van Amburgh, A.W. Bell and Y.R. Boisclair,2000166519528Fruhbeck, G., S.A. Jebb and A.M. Prentice,199818399419Gelderman, H.,197546319330Haegeman, A., A. van Zeveren and L.J. Peelman,2000317979Hamann, A. and S. Matthaei,1996104293300Moussavi, A.H., M. Ahouei, M.R., Nassiry and A. Javadmanesh,200619627631Houseknecht, K.L., C.A. Baile, R.L. Matteri and M.E. Spurlock,19987614051420Konfortov, B.A., V.E. Licence and J.R. Miller,19991011421145Lagonigro, R., P. Wiener, F. Pilla, J.A. Woolliams and J.L. Williams,200334371374Lande, R.,198199541553Liefers, S.C., M.F.W. te Pas, R.F. Veerkamp and T. van der Lende,20028516331638Liefers, S.C., R.F. Veerkamp, M.F.W. te Pas, C. Delavaud, Y. Chilliard and T. van der lende,200386799807Lien, S., H. Sundvold, H. Klungland and D.I. Vage,199728245245Madeja, Z., T. Adamowicz, A. Chmurzynska, T. Jankowski, J. Melonek, M. Switonski and T. Strabel,20048739253927Pomp, D., T. Zou, A.C. Clutter and W. Barendse,19977514271427