Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.3356.3361Chrysanthemum morifolium Ramat. in vitro
]]>NahidJesmin SultanaShyamaliSaha KazumiHattori 1220071019An efficient and novel method was developed to initiate multiple shoots from the petal which is indispensable for normal and transgenic plant of Chrysanthemum morifolium. The study indicates that high frequency of plant regeneration that would be crucial for the application of genetic transformation methods. The 7 mm petal explants were cultured on a Murashige and Skoog`s (MS) basal nutrient medium containing plant growth regulators (cytokinin or auxin-cytokinin) with various combinations and concentrations for the study of callus formation and shoot induction. The highest callus formation (96%) in 4037 genotype cultured in MS medium supplemented with 2 mg L-1 BA and 0.1 mg L-1 NAA. The highest number of shoots was obtained from genotype 89 and 4037 in MS medium supplemented with 2 mg L-1 BA and 0.1 mg L-1 kinetin, 1 mg L-1 kinetin and 0.1 mg L-1 NAA, respectively. A significant difference in regeneration capacity was observed among five genotypes. Genotypes x growth regulators interaction caused considerable variation in the expression of regeneration responses, suggesting that determination of specific level of growth regulator concentration in the medium is necessary for a particular genotype to obtain optimum response. Strong seasonal variation in plantlet regeneration frequency was observed for every genotype. The elongated shoots were multiplied on a multiplication medium, rooted and acclimatized in a green house.]]>Bajaj, Y.P.S., M.M.S. Sidhu and A.P.S. Gill,19921992pp: 20: 69-80pp: 20: 69-80Jaehne, A., D. Becker, R. Brettschneider and H. Loerz,199489525533Ben-Jacov, J. and R.W. Langhans,1972Chrysanthemum plants by stem-tip proliferation.]]>7289290Broertjes, C., P. Koene and J.W.H. Van-Heen,1980Chrysanthemum morifolium Ram.]]>29525530Bush, S.R., E.D. Earle and R.W. Langhans,1976Chrysanthemum morifolium Indianpolis.]]>63729737Chakrabarty, D., A.K.A. Mandal and S.K. Datta,2000Dendranthema grandiflora Ramat.).]]>74293296Cleland, R.,197122197222Datta, S.K., D. Chakrabarty, M.K. Saxena, A.K.A. Mandal and A.K. Biswas, 200161373376De-Jong, J. and J.B.M. Clusters,1986in vitro culture of pedicles and petal epidermis.]]>35137148Dwivedi, A.K., B.K. Banerji, D. Chakrabarty, A.K.A. Mandal and S.K. Datta, 200070853855Fuji, Y. and K. Shimizu,1990Chrysanthemum coccineum.]]>8625627Hahn, E.J., J.H. Boe and Y.B. Lee,199839838842Da Silva, J.A.T.,20047918Kaul, V., M.R. Milier, F.J. Hutchinson and D. Richards,1990Dendrathema grandiflora Tzvelev. ]]>212130Kim, E.K., E.J. Hahn, H.N. Murthy and K.Y. Pack,200373231236Kurtyez, D.F. and H.D. Hoerl,20002315Lowe, J.M., M.R. Davey, J.B. Power and K.S. Blundy,1993Dendrathema grandiflora Tzvelev (Syn. Chrysanthemum morifolium Ramat.). ]]>33171180Malaure, R.S., G. Barclay, J.B. Power and M.R. Davey,1991Chrysanthemum morifolium using tissue culture 2, Securing natural mutations.]]>1391418Miyazaki, S. and Y. Tashiro,1978Chrysanthemum morifolium. Explant sources for stem segment culture.]]>446778Mizutani, T. and T. Tanaka,199413914Mok, D.W.S. and M.C. Mok,20015289118Mol, J.N.M., A.R. Stuitje and A. Vander-Krol,198913283294Morrish, F. and I.K. Vasil,198724431499Murashige, T. and F. Skoog,196215473497Obukosia, D.S., E. Kimani, K. Waithaka, E. Mutttu and P.M. Kimani,2005in vitro. In vitro Cell Division. ]]>41162166Rout, G.R. and P. Das,1997Chrysanthemum: A critical review.]]>69239257Tanaka, K., Y. Kanno, S. Kudo and M. Suzuki,199867249249